CEP164 Polyclonal antibody

CEP164 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, dog and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22227-1-AP

Synonyms

centrosomal protein 164kDa, Centrosomal protein of 164 kDa, CEP164, KIAA1052



Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inRPE1 cells
Positive IHC detected inhuman colon tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22227-1-AP is CEP164 Fusion Protein expressed in E. coli.

Tested Reactivity human, dog
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEP164 fusion protein Ag17570
Full Name centrosomal protein 164kDa
Calculated molecular weight 1460 aa, 164 kDa
Observed molecular weight 164 kDa
GenBank accession numberBC000602
Gene symbol CEP164
Gene ID (NCBI) 22897
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CEP164, also named as KIAA1052 or NPHP15, is a 1460 amino acid protein, which contains 1 WW domain. CEP164 localizes in the microtubule organizing center and is expressed in several cell lines. CEP164 plays a role in microtubule organization and/or maintenance for the formation of primary cilia, a microtubule-based structure that protrudes from the surface of epithelial cells. CEP164 plays a critical role in G2/M checkpoint and nuclear divisions. The expression of CEP164 is normally limited to the mother centriole, and CEP164 can be used as a useful marker for mother centriole.

Protocols

Product Specific Protocols
WB protocol for CEP164 antibody 22227-1-APDownload protocol
IHC protocol for CEP164 antibody 22227-1-APDownload protocol
IF protocol for CEP164 antibody 22227-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Genes Cells

Cep128 associates with Odf2 to form the subdistal appendage of the centriole.

Authors - Hiroka Kashihara
humanIF

Nat Commun

High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy.

Authors - Mathew Bowler
humanWB

EMBO Rep

Centriolar satellites are required for efficient ciliogenesis and ciliary content regulation.

Authors - Ezgi Odabasi
humanIF

J Microsc

Expansion microscopy for the analysis of centrioles and cilia.

Authors - N Sahabandu
mouseIF

Sci Rep

NRF2-dependent gene expression promotes ciliogenesis and Hedgehog signaling.

Authors - Ana Martin-Hurtado
humanWB

J Biol Chem

The C7orf43/TRAPPC14 component links the TRAPPII complex to RABIN8 for preciliary vesicle tethering at the mother centriole during ciliogenesis.

Authors - Adrian Cuenca

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Pierrick (Verified Customer) (10-24-2019)

Great antibody for immunofluorescence on human cells. Works well in WB

  • Applications: Western Blot,Immunofluorescence,
  • Primary Antibody Dilution: CEP164 IF 1/1000 to 1/2000 and WB 1/1000
  • Cell Tissue Type: RPE1, HEK290, HeLa Kyoto