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  • KD/KO Validated

CEP55 Polyclonal antibody

CEP55 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 23891-1-AP

Synonyms

C10orf3, centrosomal protein 55kDa, Centrosomal protein of 55 kDa, CEP55, Up regulated in colon cancer 6, URCC6



Tested Applications

Positive WB detected inHEK-293 cells, human testis tissue, mouse testis tissue, rat testis tissue, Hela cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23891-1-AP is CEP55 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEP55 fusion protein Ag20940
Full Name centrosomal protein 55kDa
Calculated molecular weight 54 kDa
Observed molecular weight 47-55 kDa
GenBank accession numberBC008947
Gene symbol CEP55
Gene ID (NCBI) 55165
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CEP55, also named as C10orf3 and URCC6, is a 55 kDa protein which located in the centrosome in interphase cells and recruited to the midbody during cytokinesis, is essential for completion of cell abscission. It plays a role in mitotic exit and cytokinesis.CEP55 has two isoforms with the MW of 47 and 55 kDa.

Protocols

Product Specific Protocols
WB protocol for CEP55 antibody 23891-1-APDownload protocol
IHC protocol for CEP55 antibody 23891-1-APDownload protocol
IP protocol for CEP55 antibody 23891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Lett

CEP55 promotes cell proliferation and inhibits apoptosis via the PI3K/Akt/p21 signaling pathway in human glioma U251 cells.

Authors - Feng Li
  • KD Validated
humanWB

Biosci Trends

Lentivirus-mediated knockdown of CEP55 suppresses cell proliferation of breast cancer cells.

Authors - Ye Wang
  • KD Validated
humanIHC

Oncol Lett

Identification of key genes associated with the progression of intrahepatic cholangiocarcinoma using weighted gene co-expression network analysis.

Authors - Zi Ye
humanWB,FC

Am J Transl Res

MicroRNA-144-3p inhibits cell proliferation and induces cell apoptosis in prostate cancer by targeting CEP55.

Authors - Hao Zheng
  • KD Validated
humanWB,IHC

Cells

CEP55 Promotes Cell Motility via JAK2⁻STAT3⁻MMPs Cascade in Hepatocellular Carcinoma.

Authors - Minjing Li
  • KD Validated
humanIHC

Int J Cancer

Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.

Authors - Fangqiu Fu