Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, human testis tissue, mouse testis tissue, Jurkat cells |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23891-1-AP targets CEP55 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20940 Product name: Recombinant human CEP55 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-355 aa of BC008947 Sequence: MSSRSTKDLIKSKWGSKPSNSKSETTLEKLKGEIAHLKTSVDEITSGKGKLTDKERHRLLEKIRVLEAEKEKNAYQLTEKDKEIQRLRDQLKARYSTTALLEQLEETTREGERREQVLKALSEEKDVLKQQLSAATSRIAELESKTNTLRLSQTVAPNCFNSSINNIHEMEIQLKDALEKNQQWLVYDQQREVYVKGLLAKIFELEKKTETAAHSLPQQTKKPESEGYLQEEKQKCYNDLLASAKKDLEVERQTITQLSFELSEFRRKYEETQKEVHNLNQLLYSQRRADVQHLEDDRHKTEKIQKLREENDIARGKLEEEKKRSEELLSQVQFLYTSLLKQQEEQTRVALLEQQ Predict reactive species |
| Full Name | centrosomal protein 55kDa |
| Calculated Molecular Weight | 54 kDa |
| Observed Molecular Weight | 47-55 kDa |
| GenBank Accession Number | BC008947 |
| Gene Symbol | CEP55 |
| Gene ID (NCBI) | 55165 |
| RRID | AB_2802169 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q53EZ4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CEP55, also named as C10orf3 and URCC6, is a 55 kDa protein which located in the centrosome in interphase cells and recruited to the midbody during cytokinesis, is essential for completion of cell abscission. It plays a role in mitotic exit and cytokinesis.CEP55 has two isoforms with the MW of 47 and 55 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CEP55 antibody 23891-1-AP | Download protocol |
| IHC protocol for CEP55 antibody 23891-1-AP | Download protocol |
| IP protocol for CEP55 antibody 23891-1-AP | Download protocol |
| WB protocol for CEP55 antibody 23891-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Rabbit anti-CEP55 Polyclonal Antibody (CAT# 23891-1-AP) has 21 citations published in journals including Nature Communications, Cell Death & Disease, PLoS Biology, iScience, International Journal of Cancer, BMC Cancer, and others. Research fields cover cytokinesis, mitotic exit, and multiple cancer types including hepatocellular carcinoma, breast cancer, ovarian cancer, renal cell carcinoma, lung adenocarcinoma, melanoma, glioma, and pancreatic cancer. Applications include WB, IF, IHC, and IP.
| Species | Application | Title |
|---|---|---|
Nat Commun An oocyte meiotic midbody cap is required for developmental competence in mice | ||
J Adv Res IDR-driven LLPS of GAS2L3 scaffolds CHMP4B condensates to accelerate cytokinesis in hepatocellular carcinoma cells. | ||
Cell Death Dis iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.
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Front Immunol Harnessing multi-omics and artificial intelligence: revolutionizing prognosis and treatment in hepatocellular carcinoma.
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PLoS Biol The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization. | ||
Cells CEP55 Promotes Cell Motility via JAK2⁻STAT3⁻MMPs Cascade in Hepatocellular Carcinoma.
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Reviews
What customers say
The product has received one review with a 5-star rating. The reviewer used the antibody for Western Blot and reported that it works well at a 1:1,000 dilution. Overall, the feedback is positive, indicating reliable performance in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Nicolas (Verified Customer) (03-02-2026) | works well at 1:1,000
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