Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, human testis tissue, mouse testis tissue, Jurkat cells |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 7 publications below |
| WB | See 9 publications below |
| IHC | See 5 publications below |
| IF | See 5 publications below |
| IP | See 1 publications below |
Product Information
23891-1-AP targets CEP55 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20940 Product name: Recombinant human CEP55 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-355 aa of BC008947 Sequence: MSSRSTKDLIKSKWGSKPSNSKSETTLEKLKGEIAHLKTSVDEITSGKGKLTDKERHRLLEKIRVLEAEKEKNAYQLTEKDKEIQRLRDQLKARYSTTALLEQLEETTREGERREQVLKALSEEKDVLKQQLSAATSRIAELESKTNTLRLSQTVAPNCFNSSINNIHEMEIQLKDALEKNQQWLVYDQQREVYVKGLLAKIFELEKKTETAAHSLPQQTKKPESEGYLQEEKQKCYNDLLASAKKDLEVERQTITQLSFELSEFRRKYEETQKEVHNLNQLLYSQRRADVQHLEDDRHKTEKIQKLREENDIARGKLEEEKKRSEELLSQVQFLYTSLLKQQEEQTRVALLEQQ Predict reactive species |
| Full Name | centrosomal protein 55kDa |
| Calculated Molecular Weight | 54 kDa |
| Observed Molecular Weight | 47-55 kDa |
| GenBank Accession Number | BC008947 |
| Gene Symbol | CEP55 |
| Gene ID (NCBI) | 55165 |
| RRID | AB_2802169 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q53EZ4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CEP55, also named as C10orf3 and URCC6, is a 55 kDa protein which located in the centrosome in interphase cells and recruited to the midbody during cytokinesis, is essential for completion of cell abscission. It plays a role in mitotic exit and cytokinesis.CEP55 has two isoforms with the MW of 47 and 55 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CEP55 antibody 23891-1-AP | Download protocol |
| IHC protocol for CEP55 antibody 23891-1-AP | Download protocol |
| IP protocol for CEP55 antibody 23891-1-AP | Download protocol |
| WB protocol for CEP55 antibody 23891-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
PLoS Biol The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization. | ||
Cell Death Dis iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.
| ||
Int J Cancer Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma. | ||
Front Oncol USP9X Is Required to Maintain Cell Survival in Response to High-LET Radiation.
| ||
Cells CEP55 Promotes Cell Motility via JAK2⁻STAT3⁻MMPs Cascade in Hepatocellular Carcinoma.
| ||
iScience Non-muscle Myosin-II Is Required for the Generation of a Constriction Site for Subsequent Abscission. |
