Complement factor H Polyclonal antibody

Complement factor H Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 12748-1-AP

Synonyms

ARMD4, ARMS1, CFH, CFHL3, Complement factor H, Factor H, FH, FHL1, H factor 1, HF, HF1, HF2, HUS



Tested Applications

Positive WB detected inhuman blood
Positive IP detected inhuman plasma tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Sample-dependent, check data in validation data gallery

Product Information

12748-1-AP targets Complement factor H in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Complement factor H fusion protein Ag3482
Full Name complement factor H
Calculated molecular weight 1231 aa, 139 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC037285
Gene symbol CFH
Gene ID (NCBI) 3075
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Complement factor H (CFH) is an abundant plasma glycoprotein that regulates the function of the alternative complement pathway in fluid phase and on cellular surfaces (PMID: 2963625). Factor H binds to C3b, accelerates the decay of the alternative pathway C3-convertase (C3bBb) and acts as a cofactor for the factor I-mediated proteolytic inactivation of C3b (PMID: 15163532). It plays a critical role in the homeostasis of the complement system in plasma and in the protection of bystander host cells and tissues from damage by complement activation. Abnormalities in factor H have been associated with renal disease (PMID: 18190458).

Protocols

Product Specific Protocols
WB protocol for Complement factor H antibody 12748-1-APDownload protocol
IP protocol for Complement factor H antibody 12748-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols