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CIT Polyclonal antibody

CIT Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20033-1-AP

Synonyms

CIT, Citron Rho interacting kinase, CRIK, KIAA0949, STK21

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using CIT Polyclonal antibody (20033-1-AP)

    WB analysis of A431 using 20033-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 20033-1-AP (CIT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CIT Polyclonal antibody (20033-1-AP)

    WB analysis using 20033-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20033-1-AP (CIT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CIT Polyclonal antibody (20033-1-AP)

    WB analysis of HeLa using 20033-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20033-1-AP (CIT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CIT Polyclonal antibody (20033-1-AP)

    IHC staining of mouse brain using 20033-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20033-1-AP (CIT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CIT Polyclonal antibody (20033-1-AP)

    IHC staining of mouse brain using 20033-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20033-1-AP (CIT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CIT Polyclonal antibody (20033-1-AP)

    IF Staining of HepG2 using 20033-1-AP

    Immunofluorescent analysis of HepG2 cells, using CIT antibody 20033-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inA431 cells, HeLa cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

20033-1-AP targets CIT in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name citron (rho-interacting, serine/threonine kinase 21)
Calculated Molecular Weight 231 kDa
Observed Molecular Weight 230 kDa, 177 kDa
GenBank Accession NumberNM_007174
Gene Symbol CIT
Gene ID (NCBI) 11113
RRIDAB_10665814
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CIT, also named KIAA0949, STK21 and CRIK, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. It is required for KIF14 localization to the central spindle and midbody. CIT may play a role in cytokinesis. Putative RHO/RAC effector that binds to the GTP-bound forms of RHO and RAC1. It probably binds p21 with a tighter specificity in vivo. CIT plays an important role in the regulation of cytokinesis and the development of the central nervous system. The antibody recognizes all the isoforms of CIT.

Protocols

Product Specific Protocols
WB protocol for CIT antibody 20033-1-APDownload protocol
IHC protocol for CIT antibody 20033-1-APDownload protocol
IF protocol for CIT antibody 20033-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.

Authors - Federica Morani
View Article
mouseWB

Mol Cell Biol

E2F-2 Promotes Nuclear Condensation and Enucleation of Terminally Differentiated Erythroblasts.

Authors - Kelsey L Swartz
View Article
humanWB

Sci Rep

A three-gene signature reveals changes in the tumor immune microenvironment in the progression from NAFLD to HCC

Authors - Lijuan Liu
View Article