CNPY2, MSAP Monoclonal antibody

CNPY2, MSAP Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1G8G11

Cat no : 66173-1-Ig

Synonyms

canopy 2 homolog (zebrafish), CNPY2, CNPY2,MSAP, HP10390, MSAP, Protein canopy homolog 2, TMEM4, Transmembrane protein 4, ZSIG9



Tested Applications

Positive WB detected inA549 cells, pig liver tissue, rat liver tissue, human placenta tissue, HepG2 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66173-1-Ig targets CNPY2, MSAP in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CNPY2, MSAP fusion protein Ag6363
Full Name canopy 2 homolog (zebrafish)
Calculated molecular weight 21 kDa
Observed molecular weight 18-21 kDa
GenBank accession numberBC065015
Gene symbol CNPY2
Gene ID (NCBI) 10330
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth. It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 can stabilize MRLC and thus bring about an increase in neurite outgrowth. (PMID: 12826659 ) This antibody is specific to CNPY2

Protocols

Product Specific Protocols
WB protocol for CNPY2, MSAP antibody 66173-1-IgDownload protocol
IHC protocol for CNPY2, MSAP antibody 66173-1-IgDownload protocol
IF protocol for CNPY2, MSAP antibody 66173-1-IgDownload protocol
FC protocol for CNPY2, MSAP antibody 66173-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols