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CNPY2, MSAP Antibody 0 Publications

Mouse Monoclonal| Catalog number: 66173-1-Ig |CloneNo.: 1G8G11

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Con: 170 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, FC, ELISA
Application not tested? Please see our Guarantee


Images See all

CNPY2, MSAP Antibody FC HepG2 cells 66173-1-Ig

1X10^6 HepG2 cells were stained with 0.20ug CNPY2, MSAP antibody (66173-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH

CNPY2, MSAP Antibody WB HepG2 cells 66173-1-Ig

HepG2 cells were subjected to SDS PAGE followed by western blot with 66173-1-Ig( CNPY2, MSAP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours

CNPY2, MSAP Antibody IHC human pancreas cancer tissue 66173-1-Ig

Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 66173-1-Ig( CNPY2, MSAP antibody at dilution of 1:500 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CNPY2, MSAP Antibody IHC human pancreas cancer tissue 66173-1-Ig

Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 66173-1-Ig( CNPY2, MSAP antibody at dilution of 1:500 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CNPY2, MSAP Antibody IF HepG2 cells 66173-1-Ig

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 66173-1-Ig(CNPY2, MSAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-mouse IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
HepG2 cells, human placenta tissue, rat liver tissue
Positive IHC detected in:
human pancreas cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Positive FC detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:50-1:500
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CNPY2, MSAP fusion protein Ag6363
Full name:
canopy 2 homolog (zebrafish)
Calculated molecular weight:
21 kDa
Observed molecular weight:
21 kDa
GenBank accession number:
BC065015
Gene ID (NCBI):
10330
Gene symbol
CNPY2
Synonyms
canopy 2 homolog (zebrafish), CNPY2, CNPY2,MSAP, HP10390, MSAP, Protein canopy homolog 2, TMEM4, Transmembrane protein 4, ZSIG9

Background

CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth. It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 can stabilize MRLC and thus bring about an increase in neurite outgrowth. (PMID: 12826659 ) This antibody is specific to CNPY2


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