CNPY2/MSAP Monoclonal antibody, PBS Only

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  WB analysis using 66173-1-Ig (same clone as 66173-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66173-1-Ig (CNPY2, MSAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66173-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66173-1-Ig (same clone as 66173-1-PBS)

  WB result of CNPY2, MSAP antibody (66173-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPY2, MSAP transfected HepG2 cells. This data was developed using the same antibody clone with 66173-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 66173-1-Ig (same clone as 66173-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66173-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 66173-1-Ig (same clone as 66173-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66173-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66173-1-Ig (same clone as 66173-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66173-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66173-1-Ig (same clone as 66173-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66173-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66173-1-Ig (same clone as 66173-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66173-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66173-1-Ig (same clone as 66173-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66173-1-Ig(CNPY2, MSAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-mouse IgG(H+L). This data was developed using the same antibody clone with 66173-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CNPY2/MSAP Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66173-1-PBS

Clone No.1G8G11

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CNPY2, CNPY2, MSAP, CNPY2,MSAP, canopy 2 homolog (zebrafish), HP10390

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Product Information

66173-1-PBS targets CNPY2/MSAP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6363

  Product name: Recombinant human CNPY2,MSAP protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-182 aa of BC065015

  Sequence: MKGWGWLALLLGALLGTAWARRSQDLHCGACRALVDELEWEIAQVDPKKTIQMGSFRINPDGSQSVVEVPYARSEAHLTELLEEICDRMKEYGEQIDPSTHRKNYVRVVGRNGESSELDLQGIRIDSDISGTLKFACESIVEEYEDELIEFFSREADNVKDKLCSKRTDLCDHALHISHDEL

• Full Name: canopy 2 homolog (zebrafish)
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 18-21 kDa
• GenBank Accession Number: BC065015
• Gene Symbol: CNPY2
• Gene ID (NCBI): 10330
• RRID: AB_2881568
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y2B0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth. It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 can stabilize MRLC and thus bring about an increase in neurite outgrowth. (PMID: 12826659 ) This antibody is specific to CNPY2