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COX6B1 Polyclonal antibody

COX6B1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11425-1-AP

Synonyms

COX VIb 1, COX6B, COX6B1, COXG

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COX6B1 Polyclonal antibody (11425-1-AP)

    WB analysis using 11425-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11425-1-AP (COX6B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Caco-2 cells using COX6B1 Polyclonal antibody (11425-1-AP)

    WB analysis of Caco-2 using 11425-1-AP

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 11425-1-AP (COX6B1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using COX6B1 Polyclonal antibody (11425-1-AP)

    IHC staining of human liver cancer using 11425-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11425-1-AP (COX6B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using COX6B1 Polyclonal antibody (11425-1-AP)

    IHC staining of human liver cancer using 11425-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11425-1-AP (COX6B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using COX6B1 Polyclonal antibody (11425-1-AP)

    IHC staining of human gliomas using 11425-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11425-1-AP (COX6B1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using COX6B1 Polyclonal antibody (11425-1-AP)

    IHC staining of human gliomas using 11425-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11425-1-AP (COX6B1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using COX6B1 Polyclonal antibody (11425-1-AP)

    IHC staining of human breast cancer using 11425-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11425-1-AP (COX6B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using COX6B1 Polyclonal antibody (11425-1-AP)

    IHC staining of human breast cancer using 11425-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11425-1-AP (COX6B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using COX6B1 Polyclonal antibody (11425-1-AP)

    IF Staining of A431 using 11425-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using COX6B1 antibody (11425-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inHL-60 cells, Caco-2 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue, human gliomas tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 6 publications below

Product Information

11425-1-AP targets COX6B1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COX6B1 fusion protein Ag1984
Full Name cytochrome c oxidase subunit Vib polypeptide 1 (ubiquitous)
Calculated Molecular Weight 80 aa, 10 kDa
Observed Molecular Weight 10-13 kDa
GenBank Accession NumberBC001015
Gene Symbol COX6B1
Gene ID (NCBI) 1340
RRIDAB_2085449
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COX6B1 (Cytochrome c oxidase subunit 6B1) is also named as COX6B and belongs to the cytochrome c oxidase subunit 6B family. It connects the two COX monomers into the physiological dimeric form. Defects in COX6B1 are a cause of mitochondrial complex IV deficiency (MT-C4D).

Protocols

Product Specific Protocols
WB protocol for COX6B1 antibody 11425-1-APDownload protocol
IHC protocol for COX6B1 antibody 11425-1-APDownload protocol
IF protocol for COX6B1 antibody 11425-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Biol

PDGFRβ translocates to the nucleus and regulates chromatin remodeling via TATA element-modifying factor 1.

Authors - Natalia Papadopoulos
View Article
humanWB

Hum Reprod

Lysine glutarylation in human sperm is associated with progressive motility.

Authors - Yi-Min Cheng
View Article
human

Hum Reprod

Posttranslational lysine 2-hydroxyisobutyrylation of human sperm tail proteins affects motility.

Authors - Yi-Min Cheng
View Article
humanWB

J Biol Chem

The cellular stress proteins CHCHD10 and MNRR1 (CHCHD2): Partners in mitochondrial and nuclear function and dysfunction.

Authors - Neeraja Purandare
View Article
humanWB

J Biol Chem

PMID: 20870728

Authors - Oliva Claudia R CR
View Article
ratWB

Mol Med Rep

Overexpression of COX6B1 protects against I/R‑induced neuronal injury in rat hippocampal neurons.

Authors - Shan Yang
View Article