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CPS1 Polyclonal antibody

CPS1 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18703-1-AP

Synonyms

CPS1, CPSase I



Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

18703-1-AP targets CPS1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name carbamoyl-phosphate synthetase 1, mitochondrial
Calculated molecular weight 165 kDa
Observed molecular weight 116 kDa, 165 kDa
GenBank accession numberBC140943
Gene symbol CPS1
Gene ID (NCBI) 1373
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CPS1 is an enzyme that catalyzes the first committed step of the hepatic urea cycle, which is important in the removal of excess urea from cells. Expressed primarily in the liver and small intestine, CPS1 can be used as a marker for mitochondria in these tissues. In addition, CPS1 expression appears to be lost in adenocarcinomas of the small intestine. The antibody can recognize two isoforms of this gene around 165 kDa and 116 kDa.

Protocols

Product Specific Protocols
WB protocol for CPS1 antibody 18703-1-APDownload protocol
IF protocol for CPS1 antibody 18703-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Proteome Res

Significant Down-Regulation of Urea Cycle Generates Clinically Relevant Proteomic Signature in Hepatocellular Carcinoma Patients with Macrovascular Invasion.

Authors - Yin Cao
humanWB

J Cell Mol Med

Caspase recruitment domain family member 10 regulates carbamoyl phosphate synthase 1 and promotes cancer growth in bladder cancer cells.

Authors - Xi Liu
  • KD Validated
humanWB

Methods Mol Biol

Proteomics analysis of human nonalcoholic fatty liver.

Authors - Rodriguez-Suarez Eva E
humanWB

Biomolecules

Carbamoyl-Phosphate Synthase 1 as a Novel Target of Phomoxanthone A, a Bioactive Fungal Metabolite.

Authors - Sara Ceccacci
humanWB

Mol Genet Metab Rep

Therapeutic effect of N-carbamylglutamate in CPS1 deficiency.

Authors - Yohei Sugiyama
WB

Gene

Studies on the biological functions of CPS1 in AFB1 induced hepatocarcinogenesis.

Authors - Chi Yang