CPT1A Monoclonal antibody

CPT1A Monoclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

5D8G9

Cat no : 66039-1-Ig

Synonyms

CPT I, CPT1, CPT1 L, CPT1A, CPTI L, L CPT1



Tested Applications

Positive WB detected inA549 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66039-1-Ig is CPT1A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CPT1A fusion protein Ag7745
Full Name carnitine palmitoyltransferase 1A (liver)
Calculated molecular weight 88 kDa
Observed molecular weight 86 kDa
GenBank accession numberBC000185
Gene symbol CPT1A
Gene ID (NCBI) 1374
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.

Protocols

Product Specific Protocols
WB protocol for CPT1A antibody 66039-1-IgDownload protocol
IHC protocol for CPT1A antibody 66039-1-IgDownload protocol
IP protocol for CPT1A antibody 66039-1-IgDownload protocol
FC protocol for CPT1A antibody 66039-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Mol Biosyst

Metabolomics reveal mitochondrial and fatty acid metabolism disorders that contribute to the development of DKD in T2DM patients.

Authors - Ling Li
mouseWB,IHC

PLoS One

Circadian and Dopaminergic Regulation of Fatty Acid Oxidation Pathway Genes in Retina and Photoreceptor Cells.

Authors - Patrick Vancura
mouseWB

Food Funct

PMID: 32613952

Authors - Shuli Man

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Mohammad (Verified Customer) (04-29-2019)

working well, Without any non-specific band

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse hippocampus