CSNK1A1 Polyclonal antibody
CSNK1A1 Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 55192-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MCF7 cells, HeLa cells, NIH/3T3 cells |
Positive IHC detected in | human breast cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | A549 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 2 publications below |
Product Information
55192-1-AP targets CSNK1A1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | casein kinase 1, alpha 1 |
Calculated molecular weight | 42 kDa |
Observed molecular weight | 42-45 kDa |
GenBank accession number | NM_001892 |
Gene symbol | CSNK1A1 |
Gene ID (NCBI) | 1452 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
CSNK1A1, also named as CK1, belongs to the protein kinase superfamily, CK1 Ser/Thr protein kinase family and Casein kinase I subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. CSNK1A1 can phosphorylate a large number of proteins. It participates in Wnt signaling. CSNK1A1 phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis. This antibody is specific to CSNK1A1.
Protocols
Product Specific Protocols | |
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WB protocol for CSNK1A1 antibody 55192-1-AP | Download protocol |
IHC protocol for CSNK1A1 antibody 55192-1-AP | Download protocol |
IF protocol for CSNK1A1 antibody 55192-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Osteoporos Int Differentially expressed proteins identified by TMT proteomics analysis in bone marrow microenvironment of osteoporotic patients. | ||
Cell Signal Fam83d modulates MAP kinase and AKT signaling and is induced during neurogenic skeletal muscle atrophy. |