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CSNK1A1 Polyclonal antibody

CSNK1A1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55192-1-AP

Synonyms

casein kinase 1, alpha 1, Casein kinase I isoform alpha, CK1, CKI alpha, CSNK1A1, HLCDGP1, PRO2975

Validation Data Gallery

  • WB analysis of MCF-7 using 55192-1-AP

    WB analysis of MCF-7 using 55192-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 55192-1-AP (CSNK1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 55192-1-AP

    IHC staining of human breast cancer using 55192-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55192-1-AP (CSNK1A1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 55192-1-AP

    IHC staining of human breast cancer using 55192-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55192-1-AP (CSNK1A1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 55192-1-AP

    IF Staining of A549 using 55192-1-AP

    Immunofluorescent analysis of A549 cells using 55192-1-AP (CSNK1A1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMCF7 cells, HeLa cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

55192-1-AP targets CSNK1A1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name casein kinase 1, alpha 1
Calculated molecular weight 42 kDa
Observed molecular weight 42-45 kDa
GenBank accession numberNM_001892
Gene symbol CSNK1A1
Gene ID (NCBI) 1452
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

CSNK1A1, also named as CK1, belongs to the protein kinase superfamily, CK1 Ser/Thr protein kinase family and Casein kinase I subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. CSNK1A1 can phosphorylate a large number of proteins. It participates in Wnt signaling. CSNK1A1 phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis. This antibody is specific to CSNK1A1.

Protocols

Product Specific Protocols
WB protocol for CSNK1A1 antibody 55192-1-APDownload protocol
IHC protocol for CSNK1A1 antibody 55192-1-APDownload protocol
IF protocol for CSNK1A1 antibody 55192-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Osteoporos Int

Differentially expressed proteins identified by TMT proteomics analysis in bone marrow microenvironment of osteoporotic patients.

Authors - Q Zhou
View Article
humanWB

Cell Signal

Fam83d modulates MAP kinase and AKT signaling and is induced during neurogenic skeletal muscle atrophy.

Authors - Lisa M Cooper
View Article