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  • KD/KO Validated

CSNK1A1 Polyclonal antibody

CSNK1A1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 55192-1-AP

Synonyms

casein kinase 1, alpha 1, Casein kinase I isoform alpha, CK1, CKI alpha, CSNK1A1, HLCDGP1, PRO2975



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55192-1-AP targets CSNK1A1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name casein kinase 1, alpha 1
Calculated Molecular Weight 42 kDa
Observed Molecular Weight30-35 kDa, 42-45 kDa
GenBank Accession NumberNM_001892
Gene Symbol CSNK1A1
Gene ID (NCBI) 1452
RRIDAB_11183034
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSNK1A1, also named as CK1, belongs to the protein kinase superfamily, CK1 Ser/Thr protein kinase family and Casein kinase I subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. CSNK1A1 can phosphorylate a large number of proteins. It participates in Wnt signaling. CSNK1A1 phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis. This antibody is specific to CSNK1A1.

Protocols

Product Specific Protocols
WB protocol for CSNK1A1 antibody 55192-1-APDownload protocol
IHC protocol for CSNK1A1 antibody 55192-1-APDownload protocol
IF protocol for CSNK1A1 antibody 55192-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Pharmacol Res

Upregulation of CSNK1A1 induced by ITGB5 confers to hepatocellular carcinoma resistance to sorafenib in vivo by disrupting the EPS15/EGFR complex

Authors - Li Gu
  • KD Validated
WB

J Cell Biol

CHMP2B regulates TDP-43 phosphorylation and cytotoxicity independent of autophagy via CK1.

Authors - Xue Deng
humanIP

Virol Sin

CK1α upregulates the IFNAR1 expression to prompt the anti-HBV effect of type I IFN in hepatoma carcinoma cells

Authors - Jing Xiong
mouseWB,IF

Stem Cell Res Ther

Nicotinamide improves in vitro lens regeneration in a mouse capsular bag model.

Authors - Xiaomin Liu
humanWB

Int J Biol Sci

Pantothenate Kinase 1 Inhibits the Progression of Hepatocellular Carcinoma by Negatively Regulating Wnt/β-catenin Signaling.

Authors - Yuyuan Zi
mouseWB,IF

Endocrinology

Casein kinase 1α regulates testosterone synthesis and testis development in adult mice

Authors - Hongzhou Guo