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CSNK1A1 Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 55192-1-AP

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Con: 68 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

CSNK1A1 Antibody WB MCF-7 cells 55192-1-AP

MCF7 cells were subjected to SDS PAGE followed by western blot with 55192-1-AP(CSNK1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

CSNK1A1 Antibody IHC human breast cancer tissue 55192-1-AP

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 55192-1-AP (CSNK1A1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CSNK1A1 Antibody IHC human breast cancer tissue 55192-1-AP

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 55192-1-AP (CSNK1A1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CSNK1A1 Antibody IF A549 cells 55192-1-AP

Immunofluorescent analysis of A549 cells using 55192-1-AP(CSNK1A1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
MCF7 cells, HeLa cells, NIH/3T3 cells
Positive IHC detected in:
human breast cancer tissue, human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
A549 cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:50-1:500
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
casein kinase 1, alpha 1
Calculated molecular weight:
42 kDa
Observed molecular weight:
42-45 kDa
GenBank accession number:
NM_001892
Gene ID (NCBI):
1452
Gene symbol
CSNK1A1
Synonyms
casein kinase 1, alpha 1, Casein kinase I isoform alpha, CK1, CKI alpha, CSNK1A1, HLCDGP1, PRO2975

Background

CSNK1A1, also named as CK1, belongs to the protein kinase superfamily, CK1 Ser/Thr protein kinase family and Casein kinase I subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. CSNK1A1 can phosphorylate a large number of proteins. It participates in Wnt signaling. CSNK1A1 phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis. This antibody is specific to CSNK1A1.


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