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Tested Applications
| Positive WB detected in | mouse testis tissue, HEK-293 |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human liver cancer tissue, human heart tissue, human placenta tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 7 publications below |
| IHC | See 2 publications below |
| IF | See 5 publications below |
Product Information
13974-1-AP targets CTNNA3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5008 Product name: Recombinant human CTNNA3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-376 aa of BC030646 Sequence: MSAETPITLNIDPQDLQVQTFTVEKLLEPLIIQVTTLVNCPQNPSSRKKGRSKRASVLLASVEEATWNLLDKGEKIAQEATVLKDELTASLEKVRKESEALKVSAERFTDDPCFLPKREAVVQAARALLAAVTRLLILADMIDVMCLLQHVSAFQRTFESLKNVANKSDLQKTYQKLGKELENLDYLAFKRQQDLKSPNQRDEIAGARASLKENSPLLHSICSACLEHSDVASLKASKDTVCEEIQNALNVISNASQGIQNMTTPPEPQAATLGSALDELENLIVLNPLTVTEEEIRPSLEKRLEAIISGAALLADSSCTRDLHRERIIAECNAIRQALQDLLSEYMNNLCNQKSDLGLTGVKIKLLEECIIFWSL Predict reactive species |
| Full Name | catenin (cadherin-associated protein), alpha 3 |
| Calculated Molecular Weight | 100 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC030646 |
| Gene Symbol | CTNNA3 |
| Gene ID (NCBI) | 29119 |
| RRID | AB_2088078 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UI47 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CTNNA3 antibody 13974-1-AP | Download protocol |
| IHC protocol for CTNNA3 antibody 13974-1-AP | Download protocol |
| IP protocol for CTNNA3 antibody 13974-1-AP | Download protocol |
| WB protocol for CTNNA3 antibody 13974-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Stem Cell An injury-responsive Rac-to-Rho GTPase switch drives activation of muscle stem cells through rapid cytoskeletal remodeling. | ||
Nat Commun Cell-cell adhesion genes CTNNA2 and CTNNA3 are tumour suppressors frequently mutated in laryngeal carcinomas.
| ||
Elife A transcriptome atlas of the mouse iris at single-cell resolution defines cell types and the genomic response to pupil dilation. | ||
Development α-Catenin-dependent cytoskeletal tension controls Yap activity in the heart.
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