Cathepsin D Polyclonal antibody

Cathepsin D Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55021-1-AP

Synonyms

Cathepsin D, CLN10, CPSD, CTSD



Tested Applications

Positive WB detected inhuman brain tissue, A431 cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

55021-1-AP targets Cathepsin D in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cathepsin D
Calculated molecular weight 45 kDa
Observed molecular weight 32 kDa
GenBank accession numberNM_001909
Gene symbol CTSD
Gene ID (NCBI) 1509
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

CTSD(Cathepsin D) is also named as CPSD,belongs to the peptidase A1 family.It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. The prepro- CTSD isoform has a molecular mass of 52 kDa, the pro-CTSD 48 kDa and the mature CTSD 32 kDa(PMID:10917609). It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.Defects in CTSD are the cause of neuronal ceroid lipofuscinosis type 10 (CLN10).

Protocols

Product Specific Protocols
WB protocol for Cathepsin D antibody 55021-1-APDownload protocol
IHC protocol for Cathepsin D antibody 55021-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols