CXCL8/IL8 Antibody

CXCL8/IL8 Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3D11D5

Cat no : 60141-2-Ig

Synonyms

C X C motif chemokine 8, CXC chemokine ligand 8, CXCL 8, CXCL8, IL 8, IL8, Inteleukin 8, interleukin 8



Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

The 60141-2-Ig immunogen is Fusion Protein CXCL8/IL8 protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CXCL8/IL8 fusion protein Ag10552
Full Name interleukin 8
Calculated molecular weight 99aa,11 kDa
Observed molecular weight
GenBank accession number BC013615
Gene symbol IL8
Gene ID (NCBI) 3576
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin 8 (IL-8), also known as CXCL8, which is a member of the CXC chemokine family. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. IL-8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL-8 is also known to be a potent promoter of angiogenesis. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression. The human IL-8 cDNA sequence predicts a protein of 99 amino acids. Removal of a 22-residue signal peptide generates a mature protein of 77 amino acids (~ 8 kDa).

Publications

SpeciesApplicationTitle
humanELISA

BMC Biotechnol

Expression, purification, and characterization of rhTyrRS.

Authors - Yongjiang Lang
humanIHC

Cancer Med

Transcriptome analysis in primary colorectal cancer tissues from patients with and without liver metastases using next-generation sequencing.

Authors - Sen Wang
humanIHC

PLoS One

Pseudomonas-Derived Ceramidase Induces Production of Inflammatory Mediators from Human Keratinocytes via Sphingosine-1-Phosphate.

Authors - Ami Oizumi
humanWB,IHC

Front Immunol

Ultraviolet B Inhibits IL-17A/TNF-α-Stimulated Activation of Human Dermal Fibroblasts by Decreasing the Expression of IL-17RA and IL-17RC on Fibroblasts.

Authors - Li Yin
humanWB

Aging Cell

Targeting amphiregulin (AREG) derived from senescent stromal cells diminishes cancer resistance and averts programmed cell death 1 ligand (PD-L1)-mediated immunosuppression.

Authors - Qixia Xu