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DDB1 Monoclonal antibody

DDB1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4F6C6

Cat no : 66010-1-Ig

Synonyms

DDB p127 subunit, DDB1, DDBA, DNA damage binding protein 1, DNA damage binding protein a, HBV X associated protein 1, UV damaged DNA binding factor, UV DDB 1, UV DDB1, XAP 1, XAP1, XPCE, XPE, XPE BF, XPE binding factor



Tested Applications

Positive WB detected inHeLa cells, A549 cells, NIH/3T3 cells, RAW 264.7 cells, MCF-7 cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue, human testis tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Sample-dependent, check data in validation data gallery

Product Information

66010-1-Ig targets DDB1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen DDB1 fusion protein Ag17903
Full Name damage-specific DNA binding protein 1, 127kDa
Calculated molecular weight 1140 aa, 127 kDa
Observed molecular weight 127 kDa
GenBank accession numberBC011686
Gene symbol DDB1
Gene ID (NCBI) 1642
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDB1, also named as XAP1, XPCe, DDBa and XPE-BF, belongs to the DDB1 family. It is required for DNA repair. DDB1 binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. This antibody is specific to DDB1.

Protocols

Product Specific Protocols
WB protocol for DDB1 antibody 66010-1-IgDownload protocol
IHC protocol for DDB1 antibody 66010-1-IgDownload protocol
IF protocol for DDB1 antibody 66010-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Virol

Degradation of SAMHD1 by Vpx is Independent of Uncoating.

Authors - Paula Jáuregui
  • KD Validated