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DDX19A Polyclonal antibody

DDX19A Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15209-1-AP

Synonyms

DDX19 DDX19L, DDX19 like protein, DDX19A, DDX19L, DEAD box protein 19A

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using DDX19A Polyclonal antibody (15209-1-AP)

    WB analysis using 15209-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15209-1-AP (DDX19A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using DDX19A Polyclonal antibody (15209-1-AP)

    IHC staining of human ovary tumor using 15209-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15209-1-AP (DDX19A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using DDX19A Polyclonal antibody (15209-1-AP)

    IHC staining of human ovary tumor using 15209-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15209-1-AP (DDX19A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, K-562 cells, HeLa cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15209-1-AP targets DDX19A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX19A fusion protein Ag7362
Full Name DEAD (Asp-Glu-Ala-As) box polypeptide 19A
Calculated Molecular Weight 54 kDa
Observed Molecular Weight44 kDa and 54 kDa
GenBank Accession NumberBC005162
Gene Symbol DDX19A
Gene ID (NCBI) 55308
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX19A, also known as DDX19L, belongs to the DEAD-box helicase family. DDX19A was identified as a novel cytosolic RNA sensor that could activate the NLRP3 inflammasome during virus infection. In addition, DDX19A was proven to be associated with NADPH oxidase 1 (NOX1)-mediated oxidative stress in tumor necrosis factor (TNF)-α-induced A549 cell (PMID: 33968728). DDX19A has 2 isoforms with the molecular mass of 44 and 54 kDa. DDX19A may represent biomarkers of metastasis and novel therapeutic targets in CSCC patients.

Protocols

Product Specific Protocols
WB protocol for DDX19A antibody 15209-1-APDownload protocol
IHC protocol for DDX19A antibody 15209-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Immunol

DDX19A Senses Viral RNA and Mediates NLRP3-Dependent Inflammasome Activation.

Authors - Jiangnan Li
View Article