DGCR8 Monoclonal antibody

DGCR8 Monoclonal Antibody for IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

4D11A3

Cat no : 60084-1-Ig

Synonyms

C22orf12, DGCR8, DGCRK6, Gy1



Tested Applications

Positive WB detected inK562 cells, HepG2 cells, BxPC-3 cells, human brain tissue, HEK-293 cells, RAW 264.7 cells, A431 cells
Positive IP detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

60084-1-Ig targets DGCR8 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DGCR8 fusion protein Ag4871
Full Name DiGeorge syndrome critical region gene 8
Calculated molecular weight 773 aa, 86 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC009323
Gene symbol DGCR8
Gene ID (NCBI) 54487
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa.

Protocols

Product Specific Protocols
WB protocol for DGCR8 antibody 60084-1-IgDownload protocol
IP protocol for DGCR8 antibody 60084-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Neurochem

Primary microRNA precursor transcripts are localized at post-synaptic densities in adult mouse forebrain.

Authors - Lugli Giovanni G
humanWB

Oncogene

Identification of microprocessor-dependent cancer cells allows screening for growth-sustaining micro-RNAs.

Authors - Peric D D
human, mouseWB

Nucleic Acids Res

SUMOylation at K707 of DGCR8 controls direct function of primary microRNA.

Authors - Changhong Zhu