- Featured Product
- KD/KO Validated
DIABLO Polyclonal Antibody for IHC, IP, WB, ELISA
Cat no : 10434-1-AP
DIABLO, diablo homolog (Drosophila), Diablo homolog, mitochondrial, DIABLO S, SMAC, SMAC3
Validation Data Gallery
|Positive WB detected in||mouse testis tissue, HEK-293 cells, HeLa cells, Jurkat cells, HepG2 cells, Sp2/0 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human testis tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10434-1-AP targets DIABLO in IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.
|Reactivity||human, mouse, rat|
|Cited Species||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DIABLO fusion protein Ag0696|
|Full Name||diablo homolog (Drosophila)|
|Calculated molecular weight||27 kDa|
|Observed molecular weight||20 kDa|
|GenBank accession number||BC011909|
|Gene ID (NCBI)||56616|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20oC. Aliquoting is unnecessary for -20oC storage.|
DIABLO, also named as SMAC, promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. DIABLO inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. This antibody recognizes all the three isoforms (27 kDa and 22 kDa) of DIABLO. The highest expression was in the testis, adrenal gland, and ears.
Med Sci Monit
Resveratrol Reduces Oxidative Stress and Apoptosis in Podocytes via Sir2-Related Enzymes, Sirtuins1 (SIRT1)/Peroxisome Proliferator-Activated Receptor γ Co-Activator 1α (PGC-1α) Axis.
Phloretin induces apoptosis of human esophageal cancer via a mitochondria-dependent pathway.
Acta Biochim Biophys Sin (Shanghai)
Cathelicidin PR-39 peptide inhibits hypoxia/reperfusion-induced kidney cell apoptosis by suppression of the endoplasmic reticulum-stress pathway.
Shikonin and 4-hydroxytamoxifen synergistically inhibit the proliferation of breast cancer cells through activating apoptosis signaling pathway in vitro and in vivo.
J Cell Physiol
Resveratrol ameliorates podocyte damage in diabetic mice via SIRT1/PGC-1α mediated attenuation of mitochondrial oxidative stress.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
SHUBHAM DUBEY (03-14-2019)