DNMT3A Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 20954-1-AP

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Con: 35 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
HEK-293 cells, HepG2 cells, Jurkat cells, MCF-7 cells, mouse testis tissue, NIH/3T3 cells, PC-3 cells

Positive IP detected in:
HeLa cells

ApplicationNote:
Recommended for detection of DNMT3A by WB test

Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
DNA (cytosine-5-)-methyltransferase 3 alpha

Calculated molecular weight:
912aa,102 kDa

Observed molecular weight:
120-130 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
DNMT3A

Synonyms
DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, DNMT3A, DNMT3A2, M.HsaIIIA
Background

DNA methylation in vertebrate animals is an epigenetic modification that is important for embryonic development, imprinting, and the inactivation of X chromosomes. DNA methylation is catalyzed by a family of DNA methyltransferases (DNMTs) that include the maintenance enzyme DNMT1 and de novo methyltransferases DNMT3a and DNMT3b. The overexpression of DNMT1, DNMT3a, and DNMT3b has been reported in various malignancies, including gastric, urothelial, and lung cancers, and may be related to tumorigenesis, tumor progression, and poor survival. Two isoforms of DNMT3a exist: the full-length DNMT3a, and the shorter form DNMT3a2 which lacks the N-terminal fragment. DNMT3a is expressed ubiquitously at low levels, while DNMT3a2 is specially expressed at high levels in embryonic stem cells and shows restricted expression in tissues known to undergo de novo methylation including testis and ovary. This antibody was raised against the N-terminal region of human DNMT3a. It is expected to detect the 120-130 kDa DNMT3a but not 72-100 kDa DNMT3a2.


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