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DPP4/CD26 Polyclonal antibody

DPP4/CD26 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, hamster and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10940-1-AP

Synonyms

ADABP, ADCP 2, ADCP2, CD26, dipeptidyl peptidase 4, Dipeptidyl peptidase IV, DPP IV, DPP4, DPPIV, T cell activation antigen CD26, TP103



Tested Applications

Positive WB detected inPC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10940-1-AP targets DPP4/CD26 in WB, IHC, IF, ELISA applications and shows reactivity with human, hamster samples.

Tested Reactivity human, hamster
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DPP4/CD26 fusion protein Ag1380
Full Name dipeptidyl-peptidase 4
Calculated molecular weight 88 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC013329
Gene symbol DPP4
Gene ID (NCBI) 1803
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DPP4 (also known as CD26) is a serine exopeptidase that cleaves X-proline dipeptides from the N terminus of polypeptides. It is an intrinsic membrane glycoprotein anchored into the cell membrane by its N-terminal end. High levels of the enzyme are found in the brush-border membranes of the kidney proximal tubule and of the small intestine, but several other tissues also express the enzyme. The enzyme is present in the fetal colon but disappears at birth. It is ectopically expressed in some human colon adenocarcinomas and human colon cancer cell lines(PMID:1977364).The dimeric 150- 220 kDa DPPIV has been reported to be active and accessible to DFP labeling, but the 110 kDa monomeric DPPIV is not(PMID:9065413). Sometimes traces of the 290 kDa active dimeric form of DPP IV as well as a 55-60 kDa protein appeared in the immunopurified DPP IV preparation. N-terminal amino acid sequence analysis revealed that the 55-60 kDa protein represents a fragment of DPP IV starting at amino acid 28.(PMID:9654125).

Protocols

Product Specific Protocols
WB protocol for DPP4/CD26 antibody 10940-1-APDownload protocol
IHC protocol for DPP4/CD26 antibody 10940-1-APDownload protocol
IF protocol for DPP4/CD26 antibody 10940-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Obesity (Silver Spring)

DPP4 Inhibition attenuates filtration barrier injury and oxidant stress in the zucker obese rat.

Authors - Ravi Nistala
ratIHC

Exp Ther Med

Effects of activated carbon N-acetylcysteine sustained-release microcapsule on dipeptidyl peptidase IV expression in young rats with non-alcoholic fatty liver disease.

Authors - Hongping Zhou
ratIF

J Agric Food Chem

A Low ω-6/ω-3 Ratio High-Fat Diet Improves Rat Metabolism via Purine and Tryptophan Metabolism in the Intestinal Tract, While Reversed by Inulin.

Authors - Yao Xiao
ratWB

Endocrinology

Dipeptidylpeptidase inhibition is associated with improvement in blood pressure and diastolic function in insulin-resistant male Zucker obese rats.

Authors - Aroor Annayya R AR
human,ratWB,IF,IHC

Biochem Pharmacol

Dipeptidyl peptidase-4 inhibition improves endothelial senescence by activating AMPK/SIRT1/Nrf2 signaling pathway.

Authors - Zhihui Chen
  • KD Validated
ratWB

Hypertension

Dipeptidyl peptidase IV regulates proliferation of preglomerular vascular smooth muscle and mesangial cells.

Authors - Jackson Edwin K EK