Tested Applications
| Positive IHC detected in | mouse brain tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22022-1-AP targets DRD2 in IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16821 Product name: Recombinant human DRD2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 200-384 aa of BC021195 Sequence: VPFIVTLLVYIKIYIVLRRRRKRVNTKRSSRAFRAHLRAPLKGNCTHPEDMKLCTVIMKSNGSFPVNRRRVEAARRAQELEMEMLSSTSPPERTRYSPIPPSHHQLTLPDPSHHGLHSTPDSPAKPEKNGHAKDHPKIAKIFEIQTMPNGKTRTSLKTMSRRKLSQQKEKKATQMLAIVLGVFII Predict reactive species |
| Full Name | dopamine receptor D2 |
| Calculated Molecular Weight | 443 aa, 51 kDa |
| GenBank Accession Number | BC021195 |
| Gene Symbol | DRD2 |
| Gene ID (NCBI) | 1813 |
| RRID | AB_2878972 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P14416 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DRD2, also named D2DR and D2R, belongs to the G-protein coupled receptor 1 family. It is one of the five types (D1 to D5) of receptors for dopamine. The activity of DRD2 is mediated by G proteins which inhibit adenylyl cyclase. Defects in DRD2 are associated with dystonia type 11 (DYT11). The antibody is specific to DRD2.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DRD2 antibody 22022-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DRD2 Polyclonal antibody (Cat# 22022-1-AP) has 2 citations. One appears in Ophthalmic Research, studying 7-methylxanthine's influence on ADORA2A-DRD2 heterodimers in human retinal pigment epithelial cells (retinal biology). The other is published in Brain and Behavior, investigating the mechanism of XuanShi Choudong Recipe in treating tic disorder via integrative network pharmacology and transcriptomics (neurology/pharmacology).
| Species | Application | Title |
|---|---|---|
Ophthalmic Res 7-Methylxanthine Influences the Behavior of ADORA2A-DRD2 Heterodimers in Human Retinal Pigment Epithelial Cells. | ||
Brain Behav Integrative Network Pharmacology and Transcriptomics Reveal the Mechanism of XuanShi Choudong Recipe in the Treatment of Tic Disorder. |
