- Featured Product
- KD/KO Validated
EHD1 Polyclonal antibody
EHD1 Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 24657-1-AP
Synonyms
Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | mouse lung tissue, mouse brain tissue, HeLa cells, mouse testis tissue, rat lung tissue |
| Positive IP detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
24657-1-AP targets EHD1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | EHD1 fusion protein Ag18400 |
| Full Name | EH-domain containing 1 |
| Calculated Molecular Weight | 534 aa, 61 kDa |
| Observed Molecular Weight | 61 kDa |
| GenBank Accession Number | BC104799 |
| Gene Symbol | EHD1 |
| Gene ID (NCBI) | 10938 |
| RRID | AB_2879658 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EHD1 antibody 24657-1-AP | Download protocol |
| IP protocol for EHD1 antibody 24657-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO J Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.
| ||
Sci Rep Rab35 and its effectors promote formation of tunneling nanotubes in neuronal cells.
| ||
Biochem Biophys Res Commun A novel CDK-independent function of p27Kip1 in preciliary vesicle trafficking during ciliogenesis. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kyosuke (Verified Customer) (06-12-2019) | I am working on thrombosis study. I use this for mouse platelet Western blot and it works very well.
|
FH Juan (Verified Customer) (05-02-2019) | It works well with a single band
|
