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  • Featured Product
  • KD/KO Validated

EHD3 Polyclonal antibody

EHD3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25320-1-AP

Synonyms

EH domain containing 3, EH domain containing protein 3, EHD2, EHD3, PAST homolog 3, PAST3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EHD3 Polyclonal antibody (25320-1-AP)

    WB analysis using 25320-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25320-1-AP (EHD3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human peripheral blood platelets using EHD3 Polyclonal antibody (25320-1-AP)

    WB analysis of human peripheral blood platelets using 25320-1-AP

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 25320-1-AP (EHD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using EHD3 Polyclonal antibody (25320-1-AP)

    WB analysis of rat brain using 25320-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 25320-1-AP (EHD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using EHD3 Polyclonal antibody (25320-1-AP)

    WB analysis of mouse kidney using 25320-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25320-1-AP (EHD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using EHD3 Polyclonal antibody (25320-1-AP)

    IHC staining of mouse lung using 25320-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 25320-1-AP (EHD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using EHD3 Polyclonal antibody (25320-1-AP)

    IHC staining of mouse lung using 25320-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 25320-1-AP (EHD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EHD3 Polyclonal antibody (25320-1-AP)

    IHC staining of mouse brain using 25320-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25320-1-AP (EHD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EHD3 Polyclonal antibody (25320-1-AP)

    IHC staining of mouse brain using 25320-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25320-1-AP (EHD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using EHD3 Polyclonal antibody (25320-1-AP)

    IHC staining of mouse kidney using 25320-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25320-1-AP (EHD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using EHD3 Polyclonal antibody (25320-1-AP)

    IHC staining of mouse kidney using 25320-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25320-1-AP (EHD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using EHD3 Polyclonal antibody (25320-1-AP)

    IHC staining of human brain using 25320-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25320-1-AP (EHD3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using EHD3 Polyclonal antibody (25320-1-AP)

    IHC staining of human brain using 25320-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25320-1-AP (EHD3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue, human peripheral blood platelets, rat brain tissue, mouse kidney tissue
Positive IHC detected inmouse lung tissue, human brain tissue, mouse brain tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25320-1-AP is EHD3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EHD3 fusion protein Ag18216
Full Name EH-domain containing 3
Calculated Molecular Weight 535 aa, 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC156996
Gene Symbol EHD3
Gene ID (NCBI) 30845
RRIDAB_2880029
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EHD3 antibody 25320-1-APDownload protocol
IHC protocol for EHD3 antibody 25320-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
zebrafishWB

Nat Cell Biol

Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.

Authors - Quanlong Lu
  • KD Validated
View Article
mouse, zebrafishWB,IF

Nat Cell Biol

Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.

Authors - Quanlong Lu
  • KD Validated
View Article
humanWB

EBioMedicine

EHD1 impairs decidualization by regulating the Wnt4/β-catenin signaling pathway in recurrent implantation failure.

Authors - Quan Zhou
View Article
humanWB

EMBO J

Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.

Authors - Tatsuo Miyamoto
  • KO Validated
View Article
ratIHC

Int J Mol Sci

Proteomic Analysis of Dysfunctional Liver Sinusoidal Endothelial Cells Reveals Substantial Differences in Most Common Experimental Models of Chronic Liver Diseases

Authors - Mar Gil
View Article
mouseIHC

Am J Physiol Renal Physiol

Unique miRNome and transcriptome profiles underlie microvascular heterogeneity in mouse kidney

Authors - Matthijs Luxen
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Kyosuke (Verified Customer) (06-12-2019)

I works well on HUVECs for Western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HUVECs