ERBB4 Polyclonal antibody

ERBB4 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19943-1-AP

Synonyms

4ICD, E4ICD, ERBB4, HER4, p180erbB4, s80HER4



Tested Applications

Positive WB detected inmouse brain tissue, MCF-7 cells, rat brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

19943-1-AP targets ERBB4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)
Calculated molecular weight 147 kDa
Observed molecular weight 180 kDa, 147 kDa
GenBank accession numberNM_001042599
Gene symbol ERBB4
Gene ID (NCBI) 2066
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ERBB4, also named as p180erbB4 and HER4, belongs to the protein kinase superfamily, Tyr protein kinase family and EGF receptor subfamily. ERBB4 specifically binds and it is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Interaction with these factors induces cell differentiation. ERBB4 is not activated by EGF, TGF-A, and amphiregulin. ERBB4 catalyze the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. The antibody recognize the C-term of ERBB4. Alternatively spliced variants which encode different protein isoforms with calculated molecular weight of 144-147 kDa have been described. The apparent molecular weight of full-length ERBB4 is 150-200 kDa due to glycosylation and phosphorylation. Full-length ERBB4 can be cleaved into an 80-kDa ERBB4 intracellular fragment and a 120-kDa extracellular fragment (PMID: 11343971; 16251361).

Protocols

Product Specific Protocols
WB protocol for ERBB4 antibody 19943-1-APDownload protocol
IHC protocol for ERBB4 antibody 19943-1-APDownload protocol
IF protocol for ERBB4 antibody 19943-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Front Cell Infect Microbiol

Transactivated Epidermal Growth Factor Receptor Recruitment of α-actinin-4 From F-actin Contributes to Invasion of Brain Microvascular Endothelial Cells by Meningitic Escherichia coli.

Authors - Jiyang Fu
humanIHC

Acta Otolaryngol

ErbB1 and ErbB2 overexpression in patients with sinonasal inverted papilloma and inverted papilloma with squamous cell carcinoma in China.

Authors - Hongbing Li
humanWB

Kaohsiung J Med Sci

MicroRNA-936/ERBB4/Akt axis exhibits anticancer properties of gastric cancer through inhibition of cell proliferation, migration, and invasion.

Authors - Shi Liu
swineWB

J Neuroinflammation

EGFR transactivation contributes to neuroinflammation in Streptococcus suis meningitis.

Authors - Xiao-Pei Yang