F2 Polyclonal antibody

F2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 24295-1-AP

Synonyms

Coagulation factor II, F2, Prothrombin, PT



Tested Applications

Positive WB detected inhuman plasma tissue
Positive IP detected inhuman plasma tissue
Positive IF detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24295-1-AP is F2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, Plasmodium falciparum
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen F2 fusion protein Ag18385
Full Name coagulation factor II (thrombin)
Calculated molecular weight 622 aa, 70 kDa
Observed molecular weight 72 kDa
GenBank accession numberBC051332
Gene symbol F2
Gene ID (NCBI) 2147
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Factor II, or prothrombin, is one of the components of this chain of plasma proteins involved in blood coagulation. Thrombin is essential in the processes of hemostasis and thrombosis.

Protocols

Product Specific Protocols
WB protocol for F2 antibody 24295-1-APDownload protocol
IF protocol for F2 antibody 24295-1-APDownload protocol
IP protocol for F2 antibody 24295-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
Plasmodium falciparumWB,IF

Malar J

The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity.

Authors - Takahiro Tougan
humanWB

Sci Rep

C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.

Authors - Anika Alberts
humanWB

Sci Rep

Molecular Camouflage of Plasmodium falciparum Merozoites by Binding of Host Vitronectin to P47 Fragment of SERA5.

Authors - Takahiro Tougan