|Positive WB detected in
|mouse liver tissue
|Positive IHC detected in
|human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:500-1:2000
|IHC : 1:100-1:400
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
20963-1-AP targets F5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
|Host / Isotype
|Rabbit / IgG
|coagulation factor V (proaccelerin, labile factor)
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
There are 13 blood coagulation factors found in the blood. There are 13 blood coagulation factors found in the blood. F5 has little or no intrinsic procoagulant activity prior to its activation through limited proteolysis by thrombin or F10a at Arg709, Arg1018 and Arg1545. The resulting F5a molecule is a heterodimer that consists of a 105 kDa heavy chain and a 71/74 kDa light chain that are noncovalently associated in a calcium-dependent manner. The intact procofactor molecule (330 kDa) as well as proteins similar in size to the factor Va light chain (74 kDa) and both light chain precursors (280 and 220 kDa) found in factor Va(IIa) were observed. And factor Xa cleaves the factor Va light chain at Arg1765 into fragments of ~30 and ~48 kDa (residues 1546-1765 and 1766-2196, respectively) in the bovine and human systems.
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