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FRS2 Polyclonal antibody

FRS2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11503-1-AP

Synonyms

FGFR signaling adaptor SNT, FGFR substrate 2, FRS2, FRS2A, FRS2alpha, SNT, SNT 1, SNT1



Tested Applications

Positive WB detected inHeLa cells, SH-SY5Y cells, human brain tissue, NIH/3T3 cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11503-1-AP targets FRS2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FRS2 fusion protein Ag2052
Full Name fibroblast growth factor receptor substrate 2
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC021562
Gene Symbol FRS2
Gene ID (NCBI) 10818
RRIDAB_2262942
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibroblast growth factor (FGF) receptor substrate 2 (FRS2) has an alternative name as SNT-1, it is an adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. FGF receptor substrates (FRS2 and FRS3) are key adaptor proteins that mediate FGF-FGFR signalling in benign as well as malignant tissue. FRS2 is a 508 amino-acid protein,which is phosphorylated on tyrosine residues. The molecular weight of non-phosphorylated FRS2 is 57-68 kDa, but phosphorylated FRS2 is 80-90 kDa. Phosphorylation of FRS2 is associated with activation of a number of MAP kinases. Allele-specific regulation of FGFR2 mRNA expression with a mildly increased breast cancer risk has been reported.

Protocols

Product Specific Protocols
WB protocol for FRS2 antibody 11503-1-APDownload protocol
IHC protocol for FRS2 antibody 11503-1-APDownload protocol
IF protocol for FRS2 antibody 11503-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Cancer Res

Transcriptomics and transposon mutagenesis identify multiple mechanisms of resistance to the FGFR inhibitor AZD4547.

Authors - Sjors M Kas
humanIHC

Cancer Res

Amplification of FRS2 and activation of FGFR/FRS2 signaling pathway in high-grade liposarcoma.

Authors - Zhang Keqiang K
mouseWB

J Med Chem

Design, Synthesis, and Biological Evaluation of Aminoindazole Derivatives as Highly Selective Covalent Inhibitors of Wild-Type and Gatekeeper Mutant FGFR4.

Authors - Min Shao
humanWB

Breast Cancer Res

FGFR1 amplification or overexpression and hormonal resistance in luminal breast cancer: rationale for a triple blockade of ER, CDK4/6, and FGFR1.

Authors - Silvana Mouron
humanWB

Genes Chromosomes Cancer

High-resolution genomic mapping reveals consistent amplification of the fibroblast growth factor receptor substrate 2 gene in well-differentiated and dedifferentiated liposarcoma.

Authors - Wang Xiaoke X
humanWB

Physiol Rep

MiR-339 inhibits proliferation of pulmonary artery smooth muscle cell by targeting FGF signaling.

Authors - Jidong Chen
  • KD Validated