GADD45A Antibody 13747-1-AP

Tested Applications

Applications:

ELISA, IP, WB
Application not tested? Please see our Guarantee

GADD45A Antibody WB PC-13 cells 13747-1-AP

PC-13 cells were subjected to SDS PAGE followed by western blot with 13747-1-AP( GADD45A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

GADD45A Antibody IP K-562 cells 13747-1-AP

IP Result of anti-GADD45A (IP:13747-1-AP, 4ug; Detection:13747-1-AP 1:500) with K-562 cells lysate 3200ug.

Species specificity:

human, mouse, rat

Positive WB detected in:

PC-13 cells, A549 cells, COLO 320 cells, K-562 cells, mouse lung tissue, mouse spleen tissue, PC-13 cells, rat lung tissue

Positive IP detected in:

K-562 cells

Recommended dilution:

WB : 1:500-1:1000

IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

GADD45A fusion protein ag4687

Full name:

growth arrest and DNA-damage-inducible, alpha

Calculated molecular weight:

165aa,18 kDa

Observed molecular weight:

18 kDa or 36 kDa

GenBank accession number:

BC011757

Gene ID (NCBI):

1647

Gene symbol

GADD45A

Synonyms

DDIT 1, DDIT1, GADD45, GADD45A

Background

GADD45A, also named as DDIT1 and GADD45, belongs to the GADD45 family. It binds to proliferating cell nuclear antigen. GADD45A may affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. GADD45A plays an essential role in active DNA demethylation during terminal osteogenic differentiation of adipose-derived mesenchymal stem cells. GADD45A has Dimer (~36 kDa) and Mono (~18 kDa) forms (PMID:11498536).

Showing 1- [2] of [2] Publications

Author:		Ji Zhou
Pubmed ID:		24981246
Journal:		Tumour Biol
Title:		Overexpression of HP1γ is associated with poor prognosis in non-small cell lung cancer cell through promoting cell survival.
Application:		WB

Species:		human

Author:		Xi Gu
Pubmed ID:		28332716
Journal:		J Cell Physiol
Title:		miR-124 and miR-9 Mediated Downregulation of HDAC5 Promotes Neurite Development Through Activating MEF2C- GPM6A Pathway.
Application:		WB

Species:		human

WB Figures

GADD45A Antibody WB A549 cells 13747-1-AP

A549 cells were subjected to SDS PAGE followed by western blot with 13747-1-AP( GADD45A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

GADD45A Antibody WB COLO 320 cells 13747-1-AP

COLO 320 cells were subjected to SDS PAGE followed by western blot with 13747-1-AP( GADD45A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

GADD45A Antibody WB K-562 cells 13747-1-AP

K-562 cells were subjected to SDS PAGE followed by western blot with 13747-1-AP( GADD45A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

GADD45A Antibody WB mouse lung tissue 13747-1-AP

mouse lung tissue were subjected to SDS PAGE followed by western blot with 13747-1-AP( GADD45A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

GADD45A Antibody WB mouse spleen tissue 13747-1-AP

mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13747-1-AP( GADD45A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

PC-13 cells were subjected to SDS PAGE followed by western blot with 13747-1-AP( GADD45A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

PC-13 cells were subjected to SDS PAGE followed by western blot with 13747-1-AP( GADD45A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

GADD45A Antibody WB rat lung tissue 13747-1-AP

rat lung tissue were subjected to SDS PAGE followed by western blot with 13747-1-AP( GADD45A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

IP Figures

IP Result of anti-GADD45A (IP:13747-1-AP, 4ug; Detection:13747-1-AP 1:500) with K-562 cells lysate 3200ug.

GADD45A Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 13747-1-AP

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Tested Applications

Product Information

Immunogen Information

Background

Showing 1- [2] of [2] Publications

WB Figures

IP Figures

GADD45A Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 13747-1-AP

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Special offer: Add control/tag antibody for $99

Tested Applications

Product Information

Immunogen Information

Background