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GAPDH Polyclonal antibody

GAPDH Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig, arabidopsis , corn , cabbage, rice and More (9)

Applications

WB, RIP, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10494-1-AP

Synonyms

G3PD, GAPD, GAPDH, OK/SW cl.12

Validation Data Gallery

Applications:
  • WB analysis using 10494-1-AP

    WB analysis using 10494-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 10494-1-AP

    WB analysis using 10494-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 10494-1-AP

    WB analysis using 10494-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 10494-1-AP

    WB analysis of mouse brain using 10494-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 10494-1-AP

    WB analysis of HEK-293 using 10494-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of Raji using 10494-1-AP

    WB analysis of Raji using 10494-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 10494-1-AP

    WB analysis of K-562 using 10494-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse heart using 10494-1-AP

    WB analysis of mouse heart using 10494-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 10494-1-AP

    WB analysis using 10494-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa cells using 10494-1-AP

    WB analysis of HeLa cells using 10494-1-AP

    Western blot of Hela cell with anti-GAPDH (10494-1-AP) at various dilutions.

  • WB analysis using 10494-1-AP

    WB analysis using 10494-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-13 using 10494-1-AP

    WB analysis of PC-13 using 10494-1-AP

    PC-13 cells were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of arabidopsis whole plant using 10494-1-AP

    WB analysis of arabidopsis whole plant using 10494-1-AP

    arabidopsis whole plant tissue were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of corn whole plant using 10494-1-AP

    WB analysis of corn whole plant using 10494-1-AP

    corn whole plant tissue were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of A549 using 10494-1-AP

    IP experiment of A549 using 10494-1-AP

    IP Result of anti-GAPDH (IP:10494-1-AP, 3ug; Detection:10494-1-AP 1:3000) with A549 cells lysate 3500ug.

  • IHC staining of human breast cancer using 10494-1-AP

    IHC staining of human breast cancer using 10494-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10494-1-AP (GAPDH antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 10494-1-AP

    IHC staining of human breast cancer using 10494-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10494-1-AP (GAPDH antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 10494-1-AP

    IHC staining of human breast cancer using 10494-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10494-1-AP (GAPDH antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 10494-1-AP

    IHC staining of human breast cancer using 10494-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10494-1-AP (GAPDH antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 10494-1-AP

    IHC staining of human lung cancer using 10494-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 10494-1-AP (GAPDH antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung cancer using 10494-1-AP

    IHC staining of human lung cancer using 10494-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 10494-1-AP (GAPDH antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 10494-1-AP

    IF Staining of HepG2 using 10494-1-AP

    Immunofluorescent analysis of HepG2 cells, using GAPDH antibody 10494-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IF Staining of HeLa using 10494-1-AP

    IF Staining of HeLa using 10494-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GAPDH antibody (10494-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

  • FC experiment of HEK-293 using 10494-1-AP

    FC experiment of HEK-293 using 10494-1-AP

    1X10^6 HEK-293 cells were stained with .2ug GAPDH antibody (10494-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-Goat anti-Rabbit IgG with dilution 1:100.

Tested Applications

Positive WB detected inhuman placenta tissue, HepG2 cells, HEK-293 cells, HeLa cells, Raji cells, K-562 cells, mouse heart tissue, PC-13 cells, arabidopsis whole plant tissue, corn whole plant tissue, mouse brain tissue, mouse skin tissue, Jurkat cells, NIH/3T3 cells, C6 cells, rat brain tissue, RAW 264.7 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:40000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

10494-1-AP targets GAPDH in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, arabidopsis , corn , cabbage, rice samples.

Tested Reactivity human, mouse, rat, pig, arabidopsis , corn , cabbage, rice
Cited Reactivityhuman, Arabidopsis, Bovine, Caenorhabditis elegans, canine, chicken, EPC cells, fish, goat, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GAPDH fusion protein Ag0766
Full Name glyceraldehyde-3-phosphate dehydrogenase
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC004109
Gene symbol GAPDH
Gene ID (NCBI) 2597
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Protocols

Product Specific Protocols
WB protocol for GAPDH antibody 10494-1-APDownload protocol
IHC protocol for GAPDH antibody 10494-1-APDownload protocol
IF protocol for GAPDH antibody 10494-1-APDownload protocol
IP protocol for GAPDH antibody 10494-1-APDownload protocol
FC protocol for GAPDH antibody 10494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Med

Carbon monoxide-releasing molecule suppresses inflammatory and osteoclastogenic cytokines in nicotine- and lipopolysaccharide-stimulated human periodontal ligament cells via the heme oxygenase-1 pathway.

Authors - Ling Song
View Article
humanWB

Bosn J Basic Med Sci

Downregulation of TSPAN13 by miR-369-3p inhibits cell proliferation in papillary thyroid cancer (PTC).

Authors - Peng Li
View Article
humanWB

BMC Pregnancy Childbirth

Screening of differentially expressed proteins from syncytiotrophoblast for severe early-onset preeclampsia in women with gestational diabetes mellitus using tandem mass tag quantitative proteomics.

Authors - Xiaotong Sun
View Article
humanWB

Cancer Sci

PMID: 30417588

Authors - Chao Qu
View Article
humanWB

Aging (Albany NY)

A CREB1/miR-433 reciprocal feedback loop modulates proliferation and metastasis in colorectal cancer.

Authors - Li Yan
View Article
humanWB

Br J Cancer

Interferon-alpha promotes immunosuppression through IFNAR1/STAT1 signalling in head and neck squamous cell carcinoma.

Authors - Hailong Ma
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Jake (Verified Customer) (11-30-2021)

GAPDH Ab works perfectly on western blot at low dilutions, will definitely buy again.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: BV-2 Cell Line
FH

Meg (Verified Customer) (02-25-2020)

It worked well for western blotting.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: THP-1
FH

Yuan (Verified Customer) (10-15-2019)

GAPDH is working very well on seminal plasma and blood plasma.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Human seminal plasma and blood plasma
FH

Hailey (Verified Customer) (10-07-2019)

This antibody is a fantastic loading control! I use this as a loading control for all my western blots where protein loading needs to be precise.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse testis
GAPDH Antibody Western Blot, validation (1:500 dilution) in Mouse testis (Cat no:10494-1-AP)
FH

Prasanth (Verified Customer) (08-19-2019)

The band is so clear, and very specific band with almost no background noise for GAPDH

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Fibroblasts
FH

Shannon (Verified Customer) (12-11-2018)

  • Applications: Cell culture,
  • Cell Tissue Type: rodent