GAPDH Antibody 564 Publications

Rabbit Polyclonal| Catalog number: 10494-1-AP

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Freight/Packing

Con: 50 μg/150 μl

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Species specificity:
human, mouse, rat, pig, arabidopsis , corn , monkey, mussel, Oriental river prawns, Sea cucumbers, silkworm, swine, tree shrews, zebrafish

Positive WB detected in:
HeLa cells,HeLa cells , HEK-293 cells, A549 cells, Jurkat cells, mouse lung tissue, PC-13 cells, Raji cells, HepG2 cells, K-562 cells, human testis tissue, MDA-MB-453s cells, mouse heart tissue, mouse kidney tissue, arabidopsis whole plant tissue, corn whole plant tissue, rat brain tissue

Positive IP detected in:
A549 cells

Positive IHC detected in:
human breast cancer tissue, human breast cancer tissue, human lung cancer tissue

Positive IF detected in:
HepG2 cells, Hela cells, HepG2 cells

Positive FC detected in:
HEK-293 cells

Recommended dilution:
WB : 1:2000-1:10000
IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
glyceraldehyde-3-phosphate dehydrogenase

Calculated molecular weight:
36 kDa

Observed molecular weight:
36 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
GAPDH

Synonyms
G3PD, GAPD, GAPDH, OK/SW cl.12
Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, RNA binding, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocated to the nucleus post modification such as S-nitrosylation. This antibody is a rabbit polyclonal antibody raised against full-length GAPDH of human origin. It can recognize the 36kDa GAPDH protein in most cells/tissues. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. GAPDH has 2 isoforms with the molecular mass of 36kDa and 32kDa.


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