KD/KO Validated

GAPDH Polyclonal antibody

GAPDH Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10494-1-AP

Publications (11290)

Reviews (13)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (8)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA

Synonyms

GAPD, Glyceraldehyde-3-phosphate dehydrogenase

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 10494-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HEK-293 using 10494-1-AP
WB result of GAPDH antibody (10494-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-GAPDH transfected HEK-293 cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 10494-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 10494-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 10494-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 10494-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse brain using 10494-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Raji using 10494-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of K-562 using 10494-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse heart using 10494-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of PC-13 using 10494-1-AP
PC-13 cells were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of arabidopsis whole plant using 10494-1-AP
arabidopsis whole plant tissue were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of corn whole plant using 10494-1-AP
corn whole plant tissue were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HEK-293 using 10494-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa cells using 10494-1-AP
Western blot of Hela cell with anti-GAPDH (10494-1-AP) at various dilutions.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of A549 using 10494-1-AP
IP result of anti-GAPDH (IP:10494-1-AP, 3ug; Detection:10494-1-AP 1:3000) with A549 cells lysate 3500ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 10494-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10494-1-AP (GAPDH antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 10494-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10494-1-AP (GAPDH antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 10494-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10494-1-AP (GAPDH antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 10494-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10494-1-AP (GAPDH antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung cancer using 10494-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 10494-1-AP (GAPDH antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung cancer using 10494-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 10494-1-AP (GAPDH antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 10494-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GAPDH antibody (10494-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 10494-1-AP
Immunofluorescent analysis of HepG2 cells, using GAPDH antibody 10494-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of HEK-293 using 10494-1-AP
1x10^6 HEK-293 cells were intracellularly stained with 0.5 ug GAPDH Polyclonal antibody (10494-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (11290)
Protocols
Reviews (13)

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MSDS SDS

Tested Applications

Positive WB detected in	human placenta tissue, Raji cells, K-562 cells, mouse heart tissue, PC-13 cells, HeLa cells, mouse brain tissue, arabidopsis whole plant tissue, corn whole plant tissue, HEK-293 cells, HepG2 cells, mouse skin tissue, RAW 264.7 cells, C6 cells, rat brain tissue, Jurkat cells, NIH/3T3 cells
Positive IP detected in	A549 cells
Positive IHC detected in	human breast cancer tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HepG2 cells
Positive FC (Intra) detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:40000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10494-1-AP targets GAPDH in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat, chicken, goat, yeast, leech, treeshrew, cavia porcellus (domestic guinea pig), arabidopsis, zebra finches
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0766 Product name: Recombinant human GAPDH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-335 aa of BC004109 Sequence: MGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE Predict reactive species
Full Name	glyceraldehyde-3-phosphate dehydrogenase
Calculated Molecular Weight	36 kDa
Observed Molecular Weight	36 kDa
GenBank Accession Number	BC004109
Gene Symbol	GAPDH
Gene ID (NCBI)	2597
RRID	AB_2263076
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P04406
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Protocols

Product Specific Protocols
FC protocol for GAPDH antibody 10494-1-AP	Download protocol
IF protocol for GAPDH antibody 10494-1-AP	Download protocol
IHC protocol for GAPDH antibody 10494-1-AP	Download protocol
IP protocol for GAPDH antibody 10494-1-AP	Download protocol
WB protocol for GAPDH antibody 10494-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The GAPDH Polyclonal antibody (catalog number 10494-1-AP) has garnered 11,284 citations published in top-tier journals including Nature, Cell, Science, Cancer Cell, Nature Metabolism, Cell Metabolism, Immunity, Nature Immunology, Gastroenterology, Autophagy, and Bone Research. These citations span a wide range of research fields, including oncology, cellular metabolism, immunology, neurodegeneration, autophagy, ferroptosis, stem cell biology, extracellular vesicle research, cardiovascular disease, aging, infectious disease, and tissue engineering, underscoring its widespread use as a reliable loading control.

Species	Application	Title
mouse,human	WB	Signal Transduct Target Ther Both IDO1 and TDO contribute to the malignancy of gliomas via the Kyn-AhR-AQP4 signaling pathway. Authors - Lisha Du View Article
mouse	WB	Signal Transduct Target Ther FGF1ΔHBS prevents diabetic cardiomyopathy by maintaining mitochondrial homeostasis and reducing oxidative stress via AMPK/Nur77 suppression. Authors - Dezhong Wang View Article
human	WB	Signal Transduct Target Ther The interferon-stimulated exosomal hACE2 potently inhibits SARS-CoV-2 replication through competitively blocking the virus entry. Authors - Junsong Zhang View Article
mouse	WB	Signal Transduct Target Ther Gefitinib and fostamatinib target EGFR and SYK to attenuate silicosis: a multi-omics study with drug exploration. Authors - Mingyao Wang View Article
human	WB	Signal Transduct Target Ther Elaiophylin triggers paraptosis and preferentially kills ovarian cancer drug-resistant cells by inducing MAPK hyperactivation Authors - Guan-Nan Li View Article
human	WB	Signal Transduct Target Ther TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion Authors - Wenwen Zheng View Article

Reviews

4.9 out of 5

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13 verified customer reviews

What customers say

All 13 reviews are highly positive (12 five-star, 1 four-star). Users consistently praise this GAPDH antibody as an excellent Western blot loading control, highlighting strong, specific bands with minimal background across mouse, human, and rat samples including cells, plasma, and tissue. It performs well over a wide dilution range (1:500–1:15,000). Several reviewers noted its flexibility for probing blots alongside other species-specific antibodies. Multiple users stated they would repurchase and recommend it for precise protein loading normalization.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Bruna (Verified Customer) (06-18-2026)	very good Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: mouse GAPDH Antibody Western Blot validation (1:1000 dilution) in mouse (Cat no:10494-1-AP)
kis (Verified Customer) (02-21-2025)	works well for WB Applications: Western Blot Primary Antibody Dilution: 1.1500 Cell Tissue Type: Human and rat liver cells
Parisa (Verified Customer) (09-26-2023)	High sensitivy and specificity. We were using a anti mouse-GAPDH antibody and this anti rabbit-GAPDH antibody gave us more flexibility for probing the blot. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: Hek293 GAPDH Antibody Western Blot validation (1:1000 dilution) in Hek293 (Cat no:10494-1-AP)
Sathyanarayani (Verified Customer) (05-12-2023)	This GADPH loading control antibody produced strong bands at 36kDa and I would definitely recommend using it for western blots. Applications: Western Blot Cell Tissue Type: Mouse lysed blood
Verena (Verified Customer) (11-09-2022)	Gives a nice, strong and specific signal in Western Blot. Applications: Western Blot Primary Antibody Dilution: 1:5000 Cell Tissue Type: Mouse
Christine (Verified Customer) (10-27-2022)	Very good loading control antibody, strong signal when used at 1:15,000 over night, can probably be diluted much more. Applications: Western Blot Primary Antibody Dilution: 1:15,000 Cell Tissue Type: HEK293T
Janelle (Verified Customer) (09-12-2022)	Perfect for WB! Applications: Western Blot Primary Antibody Dilution: 1:1000
Jake (Verified Customer) (11-30-2021)	GAPDH Ab works perfectly on western blot at low dilutions, will definitely buy again. Applications: Western Blot Primary Antibody Dilution: 1:10000 Cell Tissue Type: BV-2 Cell Line
Meg (Verified Customer) (02-25-2020)	It worked well for western blotting. Applications: Western Blot, Primary Antibody Dilution: 1:3000 Cell Tissue Type: THP-1
Yuan (Verified Customer) (10-15-2019)	GAPDH is working very well on seminal plasma and blood plasma. Applications: Western Blot, Primary Antibody Dilution: 1:2000 Cell Tissue Type: Human seminal plasma and blood plasma
Hailey (Verified Customer) (10-07-2019)	This antibody is a fantastic loading control! I use this as a loading control for all my western blots where protein loading needs to be precise. Applications: Western Blot, Primary Antibody Dilution: 1:500 Cell Tissue Type: Mouse testis GAPDH Antibody Western Blot, validation (1:500 dilution) in Mouse testis (Cat no:10494-1-AP)
Prasanth (Verified Customer) (08-19-2019)	The band is so clear, and very specific band with almost no background noise for GAPDH Applications: Western Blot, Primary Antibody Dilution: 1:1000 Cell Tissue Type: Mouse Fibroblasts
Shannon (Verified Customer) (12-11-2018)	Applications: Cell culture, Cell Tissue Type: rodent