GAPDH Antibody
GAPDH Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, pig, arabidopsis , corn and More (8)
Applications
WB, IP, IHC, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
1761
Cat no : 10494-1-AP
Synonyms
G3PD, GAPD, GAPDH, OK/SW cl.12
Validation Data Gallery
Tested Applications
| Positive WB detected in | human placenta tissue, HEK-293 cells, HeLa cells, A549 cells, PC-13 cells, arabidopsis whole plant tissue, corn whole plant tissue, rat brain tissue, Raji cells, HepG2 cells, K-562 cells, mouse heart tissue, mouse brain tissue, RAW 264.7 cells, C6 cells, mouse skin tissue |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human breast cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, Hela cells |
| Positive FC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:40000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 1570 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
10494-1-AP targets GAPDH in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, arabidopsis , corn samples.
| Tested Reactivity | human, mouse, rat, pig, arabidopsis , corn |
| Cited Reactivity | Bovine, C. elegans, Chicken, cow, dog, hamster, human, Leeches, monkey, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GAPDH fusion protein Ag0766 |
| Full Name | glyceraldehyde-3-phosphate dehydrogenase |
| Calculated molecular weight | 36 kDa |
| Observed molecular weight | 36 kDa |
| GenBank accession number | BC004109 |
| Gene symbol | GAPDH |
| Gene ID (NCBI) | 2597 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GAPDH antibody 10494-1-AP | Download protocol |
| IHC protocol for GAPDH antibody 10494-1-AP | Download protocol |
| IF protocol for GAPDH antibody 10494-1-AP | Download protocol |
| IP protocol for GAPDH antibody 10494-1-AP | Download protocol |
| FC protocol for GAPDH antibody 10494-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Mol Med Carbon monoxide-releasing molecule suppresses inflammatory and osteoclastogenic cytokines in nicotine- and lipopolysaccharide-stimulated human periodontal ligament cells via the heme oxygenase-1 pathway. | ||
Bosn J Basic Med Sci Downregulation of TSPAN13 by miR-369-3p inhibits cell proliferation in papillary thyroid cancer (PTC). | ||
BMC Pregnancy Childbirth Screening of differentially expressed proteins from syncytiotrophoblast for severe early-onset preeclampsia in women with gestational diabetes mellitus using tandem mass tag quantitative proteomics. | ||
Aging (Albany NY) A CREB1/miR-433 reciprocal feedback loop modulates proliferation and metastasis in colorectal cancer. | ||
Biochem Biophys Res Commun YY1-regulated LINC00152 promotes triple negative breast cancer progression by affecting on stability of PTEN protein. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Meg (Verified Customer) (02-25-2020) | It worked well for western blotting.
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FH Yuan (Verified Customer) (10-15-2019) | GAPDH is working very well on seminal plasma and blood plasma.
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FH Hailey (Verified Customer) (10-07-2019) | This antibody is a fantastic loading control! I use this as a loading control for all my western blots where protein loading needs to be precise.
 |
FH Prasanth (Verified Customer) (08-19-2019) | The band is so clear, and very specific band with almost no background noise for GAPDH
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FH Shannon (Verified Customer) (12-11-2018) |
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