GAPDH Antibody

GAPDH Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig, arabidopsis , corn and More (8)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10494-1-AP

Synonyms

G3PD, GAPD, GAPDH, OK/SW cl.12



Tested Applications

Positive WB detected inhuman placenta tissue, HEK-293 cells, HeLa cells, A549 cells, PC-13 cells, arabidopsis whole plant tissue, corn whole plant tissue, rat brain tissue, Raji cells, HepG2 cells, K-562 cells, mouse heart tissue, mouse brain tissue, RAW 264.7 cells, C6 cells, mouse skin tissue
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Hela cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:40000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10494-1-AP targets GAPDH in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, arabidopsis , corn samples.

Tested Reactivity human, mouse, rat, pig, arabidopsis , corn
Cited Reactivity Bovine, C. elegans, Chicken, cow, dog, hamster, human, Leeches, monkey, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GAPDH fusion protein Ag0766
Full Name glyceraldehyde-3-phosphate dehydrogenase
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession number BC004109
Gene symbol GAPDH
Gene ID (NCBI) 2597
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Protocols

Product Specific Protocols
WB protocol for GAPDH antibody 10494-1-APDownload protocol
IHC protocol for GAPDH antibody 10494-1-APDownload protocol
IF protocol for GAPDH antibody 10494-1-APDownload protocol
IP protocol for GAPDH antibody 10494-1-APDownload protocol
FC protocol for GAPDH antibody 10494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Med

Carbon monoxide-releasing molecule suppresses inflammatory and osteoclastogenic cytokines in nicotine- and lipopolysaccharide-stimulated human periodontal ligament cells via the heme oxygenase-1 pathway.

Authors - Ling Song
humanWB

Bosn J Basic Med Sci

Downregulation of TSPAN13 by miR-369-3p inhibits cell proliferation in papillary thyroid cancer (PTC).

Authors - Peng Li
humanWB

BMC Pregnancy Childbirth

Screening of differentially expressed proteins from syncytiotrophoblast for severe early-onset preeclampsia in women with gestational diabetes mellitus using tandem mass tag quantitative proteomics.

Authors - Xiaotong Sun
humanWB

Cancer Sci

PMID: 30417588

Authors - Chao Qu
humanWB

Aging (Albany NY)

A CREB1/miR-433 reciprocal feedback loop modulates proliferation and metastasis in colorectal cancer.

Authors - Li Yan
humanWB

Biochem Biophys Res Commun

YY1-regulated LINC00152 promotes triple negative breast cancer progression by affecting on stability of PTEN protein.

Authors - Xi Shen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Meg (Verified Customer) (02-25-2020)

It worked well for western blotting.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: THP-1
FH

Yuan (Verified Customer) (10-15-2019)

GAPDH is working very well on seminal plasma and blood plasma.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Human seminal plasma and blood plasma
FH

Hailey (Verified Customer) (10-07-2019)

This antibody is a fantastic loading control! I use this as a loading control for all my western blots where protein loading needs to be precise.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse testis
GAPDH Antibody Western Blot, validation (1:500 dilution) in Mouse testis (Cat no:10494-1-AP)
FH

Prasanth (Verified Customer) (08-19-2019)

The band is so clear, and very specific band with almost no background noise for GAPDH

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Fibroblasts
FH

Shannon (Verified Customer) (12-11-2018)

  • Applications: Cell culture,
  • Cell Tissue Type: rodent