- Featured Product
- KD/KO Validated
GATA1 Monoclonal antibody
GATA1 Monoclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
5E2A8
Cat no : 60011-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HL-60 cells, Raji cells, K-562 cells, Jurkat cells |
| Positive IHC detected in | human breast hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Flow Cytometry (FC) | FC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
60011-1-Ig targets GATA1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GATA1 fusion protein Ag1350 |
| Full Name | GATA binding protein 1 (globin transcription factor 1) |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | BC009797 |
| Gene Symbol | GATA1 |
| Gene ID (NCBI) | 2623 |
| RRID | AB_2294437 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GATA1 is first identified as a member of the GATA transcription factor family, whose members bind the consensus (WGATAR) binding motif [PMID:22937757]. GATA1, a zinc finger DNA-binding transcription factor, plays a critical role in the normal development of hematopoietic cell lineages. The protein contains an N-terminal region that confers transcriptional activity and a C-terminal domain that mediates binding to DNA and other factors [PMID: 8524811]. GATA-1 is also implicated in regulating the expression of the erythroid and megakaryocytic-specific genes [PMID:22937757].
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GATA1 antibody 60011-1-Ig | Download protocol |
| IHC protocol for GATA1 antibody 60011-1-Ig | Download protocol |
| FC protocol for GATA1 antibody 60011-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Blood PML4 facilitates erythroid differentiation by enhancing the transcriptional activity of GATA-1. | ||
Development The PAX-SIX-EYA-DACH network modulates GATA-FOG function in fly hematopoiesis and human erythropoiesis.
| ||
Biochem J ZNF16 (HZF1) promotes erythropoiesis and megakaryocytopoiesis via its regulation on c-KIT gene. | ||
J Cell Mol Med 1,25(OH)2 D3 blocks IFNβ production through regulating STING in epithelial layer of oral lichen planus. | ||
Cell Death Discov JAC1 suppresses proliferation of breast cancer through the JWA/p38/SMURF1/HER2 signaling. | ||
Cell Death Dis GATA3 recruits UTX for gene transcriptional activation to suppress metastasis of breast cancer. |
