GATA1 Monoclonal antibody

GATA1 Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IP, FC, ELISA

Conjugate

Unconjugated

CloneNo.

5E2A8

Cat no : 60011-1-Ig

Synonyms

ERYF1, Erythroid transcription factor, GATA 1, GATA binding factor 1, GATA1, GF 1, GF1, NF E1 DNA binding protein, NFE1, XLTT



Tested Applications

Positive WB detected inRaji cells, HL-60 cells, Jurkat cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

60011-1-Ig targets GATA1 in WB, IP, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GATA1 fusion protein Ag1350
Full Name GATA binding protein 1 (globin transcription factor 1)
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC009797
Gene symbol GATA1
Gene ID (NCBI) 2623
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GATA1 is first identified as a member of the GATA transcription factor family, whose members bind the consensus (WGATAR) binding motif [PMID:22937757]. GATA1, a zinc finger DNA-binding transcription factor, plays a critical role in the normal development of hematopoietic cell lineages. The protein contains an N-terminal region that confers transcriptional activity and a C-terminal domain that mediates binding to DNA and other factors [PMID: 8524811]. GATA-1 is also implicated in regulating the expression of the erythroid and megakaryocytic-specific genes [PMID:22937757].

Protocols

Product Specific Protocols
WB protocol for GATA1 antibody 60011-1-IgDownload protocol
FC protocol for GATA1 antibody 60011-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

GATA3 recruits UTX for gene transcriptional activation to suppress metastasis of breast cancer.

Authors - Wenqian Yu
humanWB

Biochem J

ZNF16 (HZF1) promotes erythropoiesis and megakaryocytopoiesis via its regulation on c-KIT gene.

Authors - Chen Jing J
humanIP

Blood

PML4 facilitates erythroid differentiation by enhancing the transcriptional activity of GATA-1.

Authors - Wu Jie J