CoraLite®555-conjugated GATA1 Monoclonal antibody

GATA1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

5E2A8

Cat no : CL555-60011

Synonyms

ERYF1, Erythroid transcription factor, GATA 1, GATA binding factor 1, GATA1, GF 1, GF1, NF E1 DNA binding protein, NFE1, XLTT



Tested Applications

Positive FC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 60011-1-Ig

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

CL555-60011 targets GATA1 in FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GATA1 fusion protein Ag1350
Full Name GATA binding protein 1 (globin transcription factor 1)
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC009797
Gene symbol GATA1
Gene ID (NCBI) 2623
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths555 nm / 570 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

GATA1 is first identified as a member of the GATA transcription factor family, whose members bind the consensus (WGATAR) binding motif [PMID:22937757]. GATA1, a zinc finger DNA-binding transcription factor, plays a critical role in the normal development of hematopoietic cell lineages. The protein contains an N-terminal region that confers transcriptional activity and a C-terminal domain that mediates binding to DNA and other factors [PMID: 8524811]. GATA-1 is also implicated in regulating the expression of the erythroid and megakaryocytic-specific genes [PMID:22937757].

Protocols

Product Specific Protocols
FC protocol for CL555 GATA1 antibody CL555-60011Download protocol
Standard Protocols
Click here to view our Standard Protocols