GATA1 Monoclonal antibody, PBS Only

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  WB analysis using 60011-1-Ig (same clone as 60011-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60011-1-Ig (GATA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 60011-1-Ig (same clone as 60011-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 60011-1-Ig (GATA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 60011-1-Ig (same clone as 60011-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 60011-1-Ig (same clone as 60011-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 60011-1-Ig (same clone as 60011-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 60011-1-Ig (same clone as 60011-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 60011-1-Ig (same clone as 60011-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GATA1 antibody (60011-1-Ig, Clone: 5E2A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

GATA1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 60011-1-PBS

Clone No.5E2A8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

5E2A8, ERYF1, GATA-1, GATA-binding factor 1, GF1

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Product Information

60011-1-PBS targets GATA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1350

  Product name: Recombinant human GATA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-316 aa of BC009797

  Sequence: MEFPGLGSLGTSEPLPQFVDPALVSSTPESGVFFPSGPEGLDAAASSTAPSTATAAAAALAYYRDAEAYRHSPVFQVYPLLNCMEGIPGGSPYAGWAYGKTGLYPASTVCPTREDSPPQAVEDLDGKGSTSFLETLKTERLSPDLLTLGPALPSSLPVPNSAYGGPDFSSTFFSPTGSPLNSAAYSSPKLRGTLPLPPCEARECVNCGATATPLWRRDRTGHYLCNACGLYHKMNGQNRPLIRPKKRLIVSKRAGTQCTNCQTTTTTLWRRNASGDPVCNACGLYYKLHHQHYCGGSAQLMRAQSMASRGGVVSFS

• Full Name: GATA binding protein 1 (globin transcription factor 1)
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC009797
• Gene Symbol: GATA1
• Gene ID (NCBI): 2623
• RRID: AB_2294437
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P15976
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GATA1 is first identified as a member of the GATA transcription factor family, whose members bind the consensus (WGATAR) binding motif [PMID:22937757]. GATA1, a zinc finger DNA-binding transcription factor, plays a critical role in the normal development of hematopoietic cell lineages. The protein contains an N-terminal region that confers transcriptional activity and a C-terminal domain that mediates binding to DNA and other factors [PMID: 8524811]. GATA-1 is also implicated in regulating the expression of the erythroid and megakaryocytic-specific genes [PMID:22937757].