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- KD/KO Validated
GBP5 Polyclonal antibody
GBP5 Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IP, IHC, FC, ELISA
Conjugate
Unconjugated
Cat no : 13220-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | U-937 cells, Jurkat cells |
| Positive IP detected in | U-937 cells |
| Positive IHC detected in | rat spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 31 publications below |
| IHC | See 14 publications below |
Product Information
13220-1-AP targets GBP5 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GBP5 fusion protein Ag4103 |
| Full Name | guanylate binding protein 5 |
| Calculated Molecular Weight | 586 aa, 67 kDa |
| Observed Molecular Weight | 65 kDa, 55 kDa |
| GenBank Accession Number | BC031639 |
| Gene Symbol | GBP5 |
| Gene ID (NCBI) | 115362 |
| RRID | AB_2109348 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Guanylate binding protein (GBP) 5 belongs to the GBP family, which is involved in important cellular processes, including signal transduction, translation, vesicle trafficking, and exocytosis. GBP5 was reported to be a critical cellular factor in inflammasome assembly. Furthermore, GBP5 has been reported to induce inflammatory cytokines, including IL-1 beta and IL-18, through the activation of NLRP3 and AIM2. GBP5 encodes two isoforms: GBP-5a/b (67 kDa) and GBP-5ta (55 kDa)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GBP5 antibody 13220-1-AP | Download protocol |
| IHC protocol for GBP5 antibody 13220-1-AP | Download protocol |
| IP protocol for GBP5 antibody 13220-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature Ubiquitination and degradation of GBPs by a Shigella effector to suppress host defence. | ||
Nature Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases. | ||
Cell IRGB10 Liberates Bacterial Ligands for Sensing by the AIM2 and Caspase-11-NLRP3 Inflammasomes. | ||
Nat Immunol The transcription factor IRF1 and guanylate-binding proteins target activation of the AIM2 inflammasome by Francisella infection. | ||
Nat Immunol Guanylate-binding proteins promote activation of the AIM2 inflammasome during infection with Francisella novicida.
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