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GFAP Polyclonal antibody

GFAP Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 23935-1-AP

Synonyms

GFAP



Tested Applications

Positive WB detected inmouse brain tissue, C6 cells, U-251 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23935-1-AP is GFAP Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, macaques, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GFAP fusion protein Ag20853
Full Name glial fibrillary acidic protein
Calculated molecular weight 432 aa, 50 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC013596
Gene symbol GFAP
Gene ID (NCBI) 2670
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Function

GFAP (Glial fibrillary acidic protein) is a type III intermediate filament (IF) protein specific to the central  nervous system (CNS). GFAP is one of the  main components of the intermediate filament network in astrocytes and has been proposed as playing a role in cell migration, cell motility, maintaining mechanical strength, and in mitosis.

Tissue specificity

GFAP is expressed in central nervous system cells, predominantly in astrocytes. GFAP is commonly used as an astrocyte marker.  However, GFAP is also present in peripheral glia and in non-CNS cells, including fibroblasts, chondrocytes, lymphocytes, and liver stellate cells (PMID: 21219963).

Involvement in disease
  • Mutations in GFAP lead to Alexander disease (OMIM: 203450), an autosomal dominant CNS disorder. The mutations  present in affected individuals are thought to be gain-of-function. 

  • Upregulation of GFAP is a hallmark of reactive astrocytes, in which GFAP is present in hypertrophic cellular  processes. Reactive astrogliosis is present in many neurological disorders, such as stroke, various  neurodegenerative diseases (including Alzheimer's and Parkinson's disease), and neurotrauma. 

Isoforms

Astrocytes express 10 different isoforms of GFAP that differ in the rod and tail domains (PMID: 25726916),  which means that they differ in molecular size. Isoform expression varies during the development and across different subtypes of astrocytes. Not all isoforms are upregulated in reactive astrocytes.

Post-translational modifications

Intermediate filament proteins are regulated by phosphorylation. Six phosphorylation sites have been identified in GFAP protein, at least some of which are reported to control filament assembly (PMID: 21219963).

Cellular localization

GFAP localizes to intermediate filaments and stains well in astrocyte cellular processes.

Protocols

Product Specific Protocols
WB protocol for GFAP antibody 23935-1-APDownload protocol
IHC protocol for GFAP antibody 23935-1-APDownload protocol
IF protocol for GFAP antibody 23935-1-APDownload protocol
IP protocol for GFAP antibody 23935-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Sci Rep

Label-free classification of neurons and glia in neural stem cell cultures using a hyperspectral imaging microscopy combined with machine learning.

Authors - Hiroshi Ogi
humanWB

Int J Oncol

Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.

Authors - Wei Jia
mouseIF

Biochem Biophys Res Commun

Identification and immunohistochemical characterization of G-quadruplexes in mouse brain.

Authors - Sefan Asamitsu
macaquesIF

PLoS One

Characterization of Nef expression in different brain regions of SIV-infected macaques.

Authors - Shadan S Yarandi
mouseIF

Sci Rep

Administration of molecular hydrogen during pregnancy improves behavioral abnormalities of offspring in a maternal immune activation model.

Authors - Kenji Imai