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GGH Polyclonal antibody

GGH Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13264-1-AP

Synonyms

Conjugase, Gamma Glu X carboxypeptidase, Gamma glutamyl hydrolase, GGH, GH

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GGH Polyclonal antibody (13264-1-AP)

    WB analysis using 13264-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13264-1-AP (GGH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using GGH Polyclonal antibody (13264-1-AP)

    WB analysis of MCF-7 using 13264-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 13264-1-AP (GGH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using GGH Polyclonal antibody (13264-1-AP)

    WB analysis of HepG2 using 13264-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13264-1-AP (GGH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using GGH Polyclonal antibody (13264-1-AP)

    IP experiment of HepG2 using 13264-1-AP

    IP result of anti-GGH (IP:13264-1-AP, 4ug; Detection:13264-1-AP 1:500) with HepG2 cells lysate 5600 ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using GGH Polyclonal antibody (13264-1-AP)

    IHC staining of human kidney using 13264-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13264-1-AP (GGH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using GGH Polyclonal antibody (13264-1-AP)

    IHC staining of human kidney using 13264-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13264-1-AP (GGH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHL-60 cells, MCF-7 cells, HepG2 cells, HT-1080 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13264-1-AP targets GGH in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GGH fusion protein Ag3939
Full Name gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase)
Calculated Molecular Weight 318 aa, 36 kDa
Observed Molecular Weight 30-37 kDa, 55 kDa
GenBank Accession NumberBC025025
Gene Symbol GGH
Gene ID (NCBI) 8836
RRIDAB_2877931
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GGH (Gamma glutamyl hydrolase), also named as GH or Conjugase, is a key lysosomal enzyme involved in the metabolism of folic acid and in the action of antifolate drugs (PMID: 16945597). GGH catalyzes the removal of γ-linked polyglutamates from the intracellular folylpolyglutamates to yield folylmonoglutamate coenzymes (PMID: 9614206). The full-length protein has a calculated molecular mass of 36 kDa, contains four potential asparagine glycosylation sites, and was predicted to have a 24-amino-acid signal peptide (PMID: 8816764). GGH can form homodimer which contains two potential active sites (PMID: 16945597). Some bands can be detected by SDS-PAGE: 35-37kDa (full-length), 55kDa (glycosylated form) and 30-33kDa (signal peptide removed) (PMID: 8621474).

Protocols

Product Specific Protocols
WB protocol for GGH antibody 13264-1-APDownload protocol
IHC protocol for GGH antibody 13264-1-APDownload protocol
IP protocol for GGH antibody 13264-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Front Pharmacol

Up-regulation of ABCG1 is associated with methotrexate resistance in acute lymphoblastic leukemia cells

Authors - Yao Chen
View Article
humanIHC,IF

Reprod Sci

The Identification of Gamma-Glutamyl Hydrolase in Uterine Corpus Endometrial Carcinoma: a Predictive Model and Machine Learning

Authors - Cheng Zhu
View Article