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Glutamine synthetase Antibody 9 Publications

Rabbit Polyclonal| Catalog number: 11037-2-AP

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Con: 25 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, FC, ELISA
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Images See all

Glutamine synthetase Antibody WB mouse liver tissue 11037-2-AP

mouse liver tissue were subjected to SDS PAGE followed by western blot with 11037-2-AP (Glutamine synthetase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Glutamine synthetase Antibody WB Jurkat cells 11037-2-AP

WB result of Glutamine synthetase antibody (11037-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Glutamine synthetase transfected Jurkat cells

Glutamine synthetase Antibody IF mouse brain tissue 11037-2-AP

Immunofluorescent analysis of ( 4% PFA) fixed mouse brain tissue using 11037-2-AP (Glutamine synthetase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Glutamine synthetase Antibody IHC human liver cancer tissue 11037-2-AP

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 11037-2-AP( Glutamine synthetase Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Glutamine synthetase Antibody IHC human liver cancer tissue 11037-2-AP

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 11037-2-AP( Glutamine synthetase Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Glutamine synthetase Antibody FC HepG2 cells 11037-2-AP

1X10^6 HepG2 cells were stained with 0.2ug Glutamine synthetase antibody (11037-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse liver tissue, mouse brain tissue, Jurkat cells
Positive IHC detected in:
human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
mouse brain tissue, human brain tissue
Positive FC detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:100-1:400
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Glutamine synthetase fusion protein Ag1510
Full name:
glutamate-ammonia ligase (glutamine synthetase)
Calculated molecular weight:
374aa,42 kDa
Observed molecular weight:
44 kDa, 66-70 kDa
GenBank accession number:
BC011700
Gene ID (NCBI):
2752
Gene symbol
GLUL
Synonyms
GLNS, GLUL, Glutamate ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59

Background

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts(PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). There are other bands with higher (66 kDa, 97 kDa) and lower (30 kDa)molecular weights also detected besides the 42 kDa band indicating the proteolysis of GLUL protein by the ubiquitin system(PMID:10091759).


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