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Glutamine Synthetase Polyclonal antibody
Glutamine Synthetase Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Cat no : 11037-2-AP
|Positive WB detected in||mouse liver tissue, mouse brain tissue, Jurkat cells|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue, human brain tissue|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11037-2-AP targets Glutamine Synthetase in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Glutamine Synthetase fusion protein Ag1510|
|Full Name||glutamate-ammonia ligase (glutamine synthetase)|
|Calculated molecular weight||374 aa, 42 kDa|
|Observed molecular weight||44 kDa, 66-70 kDa|
|GenBank accession number||BC011700|
|Gene ID (NCBI)||2752|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts(PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). There are other bands with higher (66 kDa, 97 kDa) and lower (30 kDa)molecular weights also detected besides the 42 kDa band indicating the proteolysis of GLUL protein by the ubiquitin system(PMID:10091759).
|Product Specific Protocols|
|WB protocol for Glutamine Synthetase antibody 11037-2-AP||Download protocol|
|IHC protocol for Glutamine Synthetase antibody 11037-2-AP||Download protocol|
|IF protocol for Glutamine Synthetase antibody 11037-2-AP||Download protocol|
|FC protocol for Glutamine Synthetase antibody 11037-2-AP||Download protocol|
|Click here to view our Standard Protocols|
J Food Biochem
Rice protein stimulates endogenous antioxidant response attributed to methionine availability in growing rats.
Evid Based Complement Alternat Med
Metabolomic Study on Nude Mice Models of Gastric Cancer Treated with Modified Si Jun Zi Tang via HILIC UHPLC-Q-TOF/MS Analysis.
J Immunol Methods
Optimisation of the quantification of glutamine synthetase and myelin basic protein in cerebrospinal fluid by a combined acidification and neutralisation protocol.
J Alzheimers Dis
Histologic subtypes of hepatoblastoma are characterized by differential canonical Wnt and Notch pathway activation in DLK+ precursors.
Elevated levels of circulating ITIH4 are associated with hepatocellular carcinoma with nonalcoholic fatty liver disease: from pig model to human study.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ryan (Verified Customer) (02-27-2019)
Tris-HCL antigen retrieval ph=9.