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  • KD/KO Validated

GSTM1 Antibody

GSTM1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 12412-1-AP

Synonyms

glutathione S transferase mu 1, GST class mu 1, GST HB subunit 4, GST1, GSTM1, GSTM1 1, GSTM1a 1a, GSTM1b 1b, GTH4, GTM1, H B, MU, MU 1



Tested Applications

Positive WB detected inmouse ovary tissue, HeLa cells, mouse kidney tissue, mouse liver tissue
Positive IHC detected inhuman gliomas tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12412-1-AP targets GSTM1 in IF, IHC, WB, ELISA applications and shows reactivity with human, mouse samples.

Reactivity human, mouse
Cited Species human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSTM1 fusion protein Ag3113
Full Name glutathione S-transferase mu 1
Calculated molecular weight 181aa,21 kDa
Observed molecular weight 27 kDa
GenBank accession number BC024005
Gene symbol GSTM1
Gene ID (NCBI) 2944
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GSTM1 antibody 12412-1-APDownload protocol
IHC protocol for GSTM1 antibody 12412-1-APDownload protocol
IF protocol for GSTM1 antibody 12412-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Arch Biochem Biophys

A physiological concentration of luteolin induces phase II drug-metabolizing enzymes through the ERK1/2 signaling pathway in HepG2 cells.

Authors - Tomoya Kitakaze
mouseWB,IHC

Sci Signal

Glutathione S-transferases promote proinflammatory astrocyte-microglia communication during brain inflammation.

Authors - Shin-Ichi Kano
  • KD Validated
mouseWB,IHC

Sci Signal

Glutathione S-transferases promote proinflammatory astrocyte-microglia communication during brain inflammation.

Authors - Shin-Ichi Kano
  • KD Validated
mouseWB

J Toxicol Pathol

Thioacetamide-induced Hepatocellular Necrosis Is Attenuated in Diet-induced Obese Mice.

Authors - Shirai Makoto M
humanWB

Autophagy

ALS-FTLD-linked mutations of SQSTM1/p62 disrupt selective autophagy and NFE2L2/NRF2 anti-oxidative stress pathway.

Authors - Zhiqiang Deng