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Tested Applications
| Positive WB detected in | mouse brain tissue, HeLa cells, mouse lung tissue, mouse heart tissue, human liver tissue, mouse kidney tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 9 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| IP | See 2 publications below |
Product Information
16814-1-AP targets HIP1R in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10353 Product name: Recombinant human HIP1R protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 266-615 aa of BC067085 Sequence: RASDMLYFKRLIQIPRLPEGPPNFLRASALAEHIKPVVVIPEEAPEDEEPENLIEISTGPPAGEPVVVADLFDQTFGPPSGSVKDDRDLQIESLKREVEMLRSELEKIKLEAQRYIAQLKSQVNALEGELEEQRKQKQKALVDNEQLRHELAQLRAAQLEGERSQGLREEAERKASATEARYNKLKEKHSELVHVHAELLRKNADTAKQLTVTQQSQEEVARVKEQLAFQVEQVKRESELKLEEKSDQLEKLKRELEAKAGELARAQEALSHTEQSKSELSSRLDTLSAEKDALSGAVRQREADLLAAQSLVRETEAALSREQQRSSQEQGELQGRLAERVWPPQMQQHH Predict reactive species |
| Full Name | huntingtin interacting protein 1 related |
| Calculated Molecular Weight | 615 aa, 70 kDa, 120 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | BC067085 |
| Gene Symbol | HIP1R |
| Gene ID (NCBI) | 9026 |
| RRID | AB_2117572 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75146 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for HIP1R antibody 16814-1-AP | Download protocol |
| IP protocol for HIP1R antibody 16814-1-AP | Download protocol |
| WB protocol for HIP1R antibody 16814-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Targeting Pin1 renders pancreatic cancer eradicable by synergizing with immunochemotherapy.
| ||
Nat Cell Biol Acetylation-dependent regulation of PD-L1 nuclear translocation dictates the efficacy of anti-PD-1 immunotherapy.
| ||
Nat Biomed Eng Inhibiting PD-L1 palmitoylation enhances T-cell immune responses against tumours. | ||
Nat Chem Biol HIP1R targets PD-L1 to lysosomal degradation to alter T cell-mediated cytotoxicity.
| ||
Sci Adv The Huntingtin-interacting protein SETD2/HYPB is an actin lysine methyltransferase.
| ||
J Clin Med HIP1R Expression and Its Association with PD-1 Pathway Blockade Response in Refractory Advanced NonSmall Cell Lung Cancer: A Gene Set Enrichment Analysis. |
