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  • KD/KO Validated

HIP1R Polyclonal antibody

HIP1R Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16814-1-AP

Synonyms

HIP 12, HIP1 related protein, HIP12, HIP1R, HIP3, ILWEQ, KIAA0655



Tested Applications

Positive WB detected inmouse brain tissue, mouse kidney tissue, human liver tissue, HeLa cells, mouse lung tissue, mouse heart tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

16814-1-AP targets HIP1R in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HIP1R fusion protein Ag10353
Full Name huntingtin interacting protein 1 related
Calculated molecular weight 615 aa, 70 kDa, 120 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC067085
Gene symbol HIP1R
Gene ID (NCBI) 9026
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HIP1R antibody 16814-1-APDownload protocol
IHC protocol for HIP1R antibody 16814-1-APDownload protocol
IP protocol for HIP1R antibody 16814-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Exp Biol Med (Maywood)

Proteomic analysis identifies NPTX1 and HIP1R as potential targets of histone deacetylase-3-mediated neurodegeneration.

Authors - Zhe Qu
humanIHC

J Clin Med

HIP1R Expression and Its Association with PD-1 Pathway Blockade Response in Refractory Advanced NonSmall Cell Lung Cancer: A Gene Set Enrichment Analysis.

Authors - Young Wha Koh
humanWB,IP,IF

Nat Chem Biol

HIP1R targets PD-L1 to lysosomal degradation to alter T cell-mediated cytotoxicity.

Authors - Huanbin Wang
  • KD Validated
humanWB

Nat Cell Biol

Acetylation-dependent regulation of PD-L1 nuclear translocation dictates the efficacy of anti-PD-1 immunotherapy.

Authors - Yang Gao
  • KO Validated