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KD/KO Validated

HIP1R Polyclonal antibody

Name: HIP1R Polyclonal antibody
Brand: Proteintech
SKU: 16814-1-AP

HIP1R Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16814-1-AP

Synonyms

HIP 12, HIP1 related protein, HIP12, HIP1R, HIP3, ILWEQ, KIAA0655

Validation Data Gallery

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AllWBIPIHC

WB analysis using 16814-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 16814-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 16814-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 16814-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 16814-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 16814-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16814-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 16814-1-AP
IP result of anti-HIP1R (IP:16814-1-AP, 4ug; Detection:16814-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
IHC staining of human placenta using 16814-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 16814-1-AP (HIP1R antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human placenta using 16814-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 16814-1-AP (HIP1R antibody) at dilution of 1:100 (under 10x lens).

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Cat No. 16814-1-AP

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Tested Applications

Positive WB detected in	mouse brain tissue, HeLa cells, mouse lung tissue, mouse heart tissue, human liver tissue, mouse kidney tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 7 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

16814-1-AP targets HIP1R in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HIP1R fusion protein Ag10353
Full Name	huntingtin interacting protein 1 related
Calculated Molecular Weight	615 aa, 70 kDa, 120 kDa
Observed Molecular Weight	120 kDa
GenBank Accession Number	BC067085
Gene Symbol	HIP1R
Gene ID (NCBI)	9026
RRID	AB_2117572
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HIP1R antibody 16814-1-AP	Download protocol
IHC protocol for HIP1R antibody 16814-1-AP	Download protocol
IP protocol for HIP1R antibody 16814-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Targeting Pin1 renders pancreatic cancer eradicable by synergizing with immunochemotherapy. Authors - Kazuhiro Koikawa KD Validated View Article
human	WB	Nat Cell Biol Acetylation-dependent regulation of PD-L1 nuclear translocation dictates the efficacy of anti-PD-1 immunotherapy. Authors - Yang Gao KO Validated View Article
human	WB	Nat Biomed Eng Inhibiting PD-L1 palmitoylation enhances T-cell immune responses against tumours. Authors - Han Yao View Article
human	WB,IP,IF	Nat Chem Biol HIP1R targets PD-L1 to lysosomal degradation to alter T cell-mediated cytotoxicity. Authors - Huanbin Wang KD Validated View Article
human	WB	Sci Adv The Huntingtin-interacting protein SETD2/HYPB is an actin lysine methyltransferase. Authors - Riyad N H Seervai KD Validated View Article
human	IHC	J Clin Med HIP1R Expression and Its Association with PD-1 Pathway Blockade Response in Refractory Advanced NonSmall Cell Lung Cancer: A Gene Set Enrichment Analysis. Authors - Young Wha Koh View Article

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HIP1R Polyclonal antibody

HIP1R Polyclonal Antibody for IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis using 16814-1-AP

WB analysis of mouse brain using 16814-1-AP

WB analysis of mouse lung using 16814-1-AP

WB analysis of mouse heart using 16814-1-AP

WB analysis of human liver using 16814-1-AP

WB analysis of mouse kidney using 16814-1-AP

WB analysis of HeLa using 16814-1-AP

IP experiment of mouse brain using 16814-1-AP

IHC staining of human placenta using 16814-1-AP

IHC staining of human placenta using 16814-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Targeting Pin1 renders pancreatic cancer eradicable by synergizing with immunochemotherapy.

Acetylation-dependent regulation of PD-L1 nuclear translocation dictates the efficacy of anti-PD-1 immunotherapy.

Inhibiting PD-L1 palmitoylation enhances T-cell immune responses against tumours.

HIP1R targets PD-L1 to lysosomal degradation to alter T cell-mediated cytotoxicity.

The Huntingtin-interacting protein SETD2/HYPB is an actin lysine methyltransferase.

HIP1R Expression and Its Association with PD-1 Pathway Blockade Response in Refractory Advanced NonSmall Cell Lung Cancer: A Gene Set Enrichment Analysis.