|Positive WB detected in||mouse brain tissue, mouse kidney tissue, human liver tissue, HeLa cells, mouse lung tissue, mouse heart tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
16814-1-AP targets HIP1R in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HIP1R fusion protein Ag10353|
|Full Name||huntingtin interacting protein 1 related|
|Calculated molecular weight||615 aa, 70 kDa, 120 kDa|
|Observed molecular weight||120 kDa|
|GenBank accession number||BC067085|
|Gene ID (NCBI)||9026|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Exp Biol Med (Maywood)
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