HLA-F Polyclonal antibody

HLA-F Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14670-1-AP

Synonyms

CDA12, HLA 5.4, HLA CDA12, HLA F, HLA F antigen, HLAF, HLA-F, Leukocyte antigen F, MHC class I antigen F



Tested Applications

Positive WB detected inRaji cells, human placenta tissue, human spleen tissue, A431 cells
Positive IHC detected inhuman tonsillitis tissue, human placenta tissue, human skin cancer tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14670-1-AP targets HLA-F in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLA-F fusion protein Ag6335
Full Name major histocompatibility complex, class I, F
Calculated molecular weight 39 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC062991
Gene symbol HLA-F
Gene ID (NCBI) 3134
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951)

Protocols

Product Specific Protocols
WB protocol for HLA-F antibody 14670-1-APDownload protocol
IHC protocol for HLA-F antibody 14670-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Surg Res

Clinical-pathological implication of human leukocyte antigen-F-positive gastric adenocarcinoma.

Authors - Ishigami Sumiya S
humanIHC

Hum Immunol

Lesion HLA-F expression is irrelevant to prognosis for patients with gastric cancer.

Authors - Zhang Jian-Gang JG
humanWB,IHC

Lung Cancer

HLA-F expression is a prognostic factor in patients with non-small-cell lung cancer.

Authors - Lin Aifen A

J Immunol

Immune Modifications in Fetal Membranes Overlying the Cervix Precede Parturition in Humans.

Authors - Louis Marcellin
humanIHC

Int J Cancer

Alteration of HLA-F and HLA I antigen expression in the tumor is associated with survival in patients with esophageal squamous cell carcinoma.

Authors - Zhang X X