|Positive WB detected in||Raji cells, human placenta tissue, human spleen tissue, A431 cells|
|Positive IHC detected in||human tonsillitis tissue, human placenta tissue, human skin cancer tissue, human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14670-1-AP targets HLA-F in WB, IHC, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||HLA-F fusion protein Ag6335|
|Full Name||major histocompatibility complex, class I, F|
|Calculated molecular weight||39 kDa|
|Observed molecular weight||40-45 kDa|
|GenBank accession number||BC062991|
|Gene ID (NCBI)||3134|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951)
J Surg Res
Clinical-pathological implication of human leukocyte antigen-F-positive gastric adenocarcinoma.
Lesion HLA-F expression is irrelevant to prognosis for patients with gastric cancer.
HLA-F expression is a prognostic factor in patients with non-small-cell lung cancer.
Immune Modifications in Fetal Membranes Overlying the Cervix Precede Parturition in Humans.
Int J Cancer
Alteration of HLA-F and HLA I antigen expression in the tumor is associated with survival in patients with esophageal squamous cell carcinoma.