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  • KD/KO Validated

HMGB2 Polyclonal antibody

HMGB2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 14597-1-AP

Synonyms

high mobility group box 2, High mobility group protein 2, High mobility group protein B2, HMG 2, HMG2, HMGB2



Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HL-60 cells, human cerebellum tissue, K-562 cells
Positive IP detected inHEK-293 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14597-1-AP targets HMGB2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HMGB2 fusion protein Ag6135
Full Name high-mobility group box 2
Calculated molecular weight 24 kDa
Observed molecular weight 33-35 kDa
GenBank accession numberBC001063
Gene symbol HMGB2
Gene ID (NCBI) 3148
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

High mobility group protein B2 (HMGB2) belongs to a family of highly conserved proteins that contain HMG box domains (11246022,14871457). All three family members (HMGB1, HMGB2, and HMGB3) contain two HMG box domains and a C-terminal acidic domain. HMGB1 is a widely expressed and highly abundant protein (14871457). HMGB2 is widely expressed during embryonic development, but it is restricted to lymphoid organs and testis in adult animals (11262228). HMGB3 is only expressed during embryogenesis (9598312). While expression varies, the biochemical properties of the different family members may be indistinguishable. The HMG box domains facilitate the binding of HMGB proteins to the minor groove of DNA, which results in local bending of the DNA double helix . HMGB proteins are recruited by and help facilitate the assembly of site-specific DNA binding proteins to their cognate binding sites in chromatin. For example, HMGB1 and HMGB2 facilitate the binding of Hox proteins, Oct proteins, p53, Rel proteins, and steroid hormone receptor proteins to their target gene promoters (11246022,14871457). Furthermore, HMGB2 interacts with RAG1 to facilitate RAG complex binding to the recombinant signal sequence (RSS) and stimulate DNA-bending and subsequent VDJ cleavage at antigen receptor genes (19317908 ,10490593). In addition to their functions in the nucleus, HMGB proteins play a significant role in extracellular signaling associated with inflammation. HMGB2 is secreted by myeloid cells and promotes proliferation and migration of endothelial cells by binding to the receptor for advanced glycation endproducts (RAGE) (19811285 ). Research studies have shown that HMGB2 overexpression in hepatocellular carcinoma is associated with poor prognosis and shorter survival time (20851854).This antibody recognizes the phosphorylation form of HMGB2 protein. The calculated molecular weight of HMGB2 is 24 kDa, but the post-modifiction of HMGB2 is about 33-35 kDa. (PMID: 18218727)

Protocols

Product Specific Protocols
WB protocol for HMGB2 antibody 14597-1-APDownload protocol
IF protocol for HMGB2 antibody 14597-1-APDownload protocol
IP protocol for HMGB2 antibody 14597-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Acta Biochim Biophys Sin (Shanghai)

Expression of HMGB2 indicates worse survival of patients and is required for the maintenance of Warburg effect in pancreatic cancer.

Authors - Xin Cai
  • KD Validated
mouseIHC

Proteome Sci

Members of the high mobility group B protein family are dynamically expressed in embryonic neural stem cells.

Authors - Abraham Ariel B AB
humanWB,IHC

Hum Pathol

HMGB2 promotes the malignancy of human gastric Cancer and indicates poor survival outcome.

Authors - Guangfei Cui
  • KD Validated
humanWB,IHC

Cell Commun Signal

HMGB2 is associated with malignancy and regulates Warburg effect by targeting LDHB and FBP1 in breast cancer.

Authors - Deyuan Fu
  • KD Validated
mouseWB

Chemosphere

Arsenic influences spermatogenesis by disorganizing the elongation of spermatids in adult male mice.

Authors - Yongli Han
ratWB

Environ Pollut

Fluoride-induced unrestored arrest during haploid period of spermatogenesis via the regulation of DDX25 in rats.

Authors - Yongli Han