A431 cells were subjected to SDS PAGE followed by western blot with 10995-1-AP(HSP70 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

A549 cells were subjected to SDS PAGE followed by western blot with 10995-1-AP(HSP70 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 10995-1-AP( HSP70 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

HepG2 cells were subjected to SDS PAGE followed by western blot with 10995-1-AP(HSP70 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

Jurkat cells were subjected to SDS PAGE followed by western blot with 10995-1-AP(HSP70 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

mouse brain tissue were subjected to SDS PAGE followed by western blot with 10995-1-AP( HSP70 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10995-1-AP (HSP70 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

mouse uterus tissue were subjected to SDS PAGE followed by western blot with 10995-1-AP(HSP70 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

WB result of 10995-1-AP(HSPA1A antibody).

PC-3 cells were subjected to SDS PAGE followed by western blot with 10995-1-AP(HSP70 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

rat brain tissue were subjected to SDS PAGE followed by western blot with 10995-1-AP( HSP70 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10995-1-AP (HSP70 antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10995-1-AP (HSP70 antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10995-1-AP( HSP70 Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10995-1-AP( HSP70 Antibody) at dilution of 1:200 (under 40x lens).

Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10995-1-AP (HSP70 antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

IP Result of anti-HSP70 (IP:10995-1-AP, 3ug; Detection:10995-1-AP 1:1000) with mouse brain tissue lysate 3600ug.

Immunofluorescent analysis of HepG2 cells, using HSPA1A antibody 10995-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

1X10^6 A549 cells were stained with 0.20ug HSP70 antibody (10995-1-AP, red) and control antibody (blue). Fixed with 90% MeOH

1X10^6 HeLa cells were stained with 0.20ug HSP70 antibody (10995-1-AP, red) and control antibody (blue). Fixed with 90% MeOH

1X10^6 HepG2 cells were stained with 0.2ug HSP70 antibody (10995-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.