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HSP70 Antibody 44 Publications

Rabbit Polyclonal| Catalog number: 10995-1-AP

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Con: 49 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, CoIP, ELISA
Application not tested? Please see our Guarantee


Images See all

HSP70 Antibody IHC human breast cancer tissue 10995-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10995-1-AP (HSP70 antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

HSP70 Antibody WB multi-cells/tissue 10995-1-AP

WB result of 10995-1-AP(HSPA1A antibody).

HSP70 Antibody WB HeLa cells 10995-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 10995-1-AP( HSP70 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

HSP70 Antibody IF HepG2 cells 10995-1-AP

Immunofluorescent analysis of HepG2 cells, using HSPA1A antibody 10995-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

HSP70 Antibody IP mouse brain tissue 10995-1-AP

IP Result of anti-HSP70 (IP:10995-1-AP, 3ug; Detection:10995-1-AP 1:1000) with mouse brain tissue lysate 3600ug.

HSP70 Antibody FC HepG2 cells 10995-1-AP

1X10^6 HepG2 cells were stained with 0.2ug HSP70 antibody (10995-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat, oysters
Positive WB detected in:
HeLa cells, multi-cells/tissue, mouse kidney tissue, mouse uterus tissue, rat brain tissue
Positive IP detected in:
mouse brain tissue
Positive IHC detected in:
human breast cancer tissue, human liver cancer tissue, mouse skin tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Positive FC detected in:
HepG2 cells, HeLa cells, A549 cells
Recommended dilution:
WB : 1:2000-1:10000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
HSP70 fusion protein Ag1446
Full name:
heat shock 70kDa protein 1A
Calculated molecular weight:
70 kDa
Observed molecular weight:
66-70 kDa
GenBank accession number:
BC009322
Gene ID (NCBI):
3303
Gene symbol
HSPA1A
Synonyms
Heat shock 70 kDa protein 1/2, heat shock 70kDa protein 1A, HSP70, HSP70 1, HSP70 1/HSP70 2, HSP70 1A, HSP70.1/HSP70.2, HSP70I, HSP72, HSPA1, HSPA1A, HSPA1B

Background

  1. What is Hsp70/HSP1A?

    HSP1A is a member of the Hsp70 (heat shock protein 70) proteins that act as molecular chaperones ensuring correct protein folding and preventing protein aggregation. Hsp70 protein production is greatly induced by various stress stimuli, including high temperature and toxins. Its expression is often elevated in various cancers.

  2. FAQs for Hsp70

    a. I cannot detect Hsp70 by western blotting

    HSP70s are typically expressed at low levels under normal physiological conditions but are dramatically up-regulated in response to cellular stress. Try to always include cell lysate from cells subjected to stress conditions as a positive control.

    b. What loading control can I use for cellular stress experiments with Hsp70?

    Choosing a loading control antibody is an important step in western blotting experimental setup. We highly recommend using more than one loading control while developing new cellular stress assays to ensure that a given treatment does not alter expression of house-keeping genes. Hsp70 has a molecular size of 70 kDa, so we recommend using GAPDH (36 kDa), actin (42 kDa), or tubulin (50-55 kDa). More information on our control antibodies can be found here: https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/.


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