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HYDIN Polyclonal antibody

HYDIN Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC,ELISA

Conjugate

Unconjugated

Cat no : 24741-1-AP

Synonyms

HYDIN, HYDIN1, HYDIN2, KIAA1864

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human testis tissue using HYDIN Polyclonal antibody (24741-1-AP)

    IHC staining of human testis using 24741-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24741-1-AP (HYDIN Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using HYDIN Polyclonal antibody (24741-1-AP)

    IHC staining of human testis using 24741-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24741-1-AP (HYDIN Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using HYDIN Polyclonal antibody (24741-1-AP)

    IHC staining of human cerebellum using 24741-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24741-1-AP (HYDIN Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using HYDIN Polyclonal antibody (24741-1-AP)

    IHC staining of human cerebellum using 24741-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24741-1-AP (HYDIN Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive IHC detected inhuman testis tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24741-1-AP is HYDIN Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HYDIN fusion protein Ag10732
Full Name hydrocephalus inducing homolog (mouse)
Calculated Molecular Weight721aa,82 kDa; 5120aa,560 kDa
GenBank Accession NumberBC028351
Gene Symbol HYDIN
Gene ID (NCBI) 54768
RRIDAB_2879700
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HYDIN (hydrocephalus inducing homolog), also named as HYDIN1 or HYDIN2, is a 5121 amino acid protein, which localizes in the cell projection HYDIN exists as seven alternatively spliced isoforms and is expressed in the ciliated ependymal cell layer of heart ventricle. HYDIN is also found in ciliated epithelial cells of developing spermatocytes, oviduct and bronchi and is required for ciliary motility. HYDIN gene mutation is involved in Ciliary dyskinesia primary 5.

Protocols

Product Specific Protocols
IHC protocol for HYDIN antibody 24741-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols