AuthentiKine™ Human IL-4 ELISA Kit
Sensitivity
1.4 pg/mL
Range
15.6-500 pg/mL
Reactivity
Human
Cat no : KE00232
Synonyms
B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, interleukin 4, Pitrakinra
Validation Data Gallery
Product Information
KE00232 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-4 ELISA kit is to be used to detect and quantify protein levels of endogenous IL-4. The assay recognizes human IL-4. An antibody specific for IL-4 has been pre-coated onto the microwells. The IL-4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IL-4 is added to detect the captured IL-4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human IL-4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 1.4 pg/mL |
| Range | 15.6-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3565 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| human plasma | 79 | 70-114 |
| Cell culture supernatants | 99 | 83-123 |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 108.93 | 6.05 | 5.6 |
| 2 | 20 | 204.09 | 11.31 | 5.5 |
| 3 | 20 | 442.97 | 13.98 | 3.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 108.82 | 3.65 | 3.4 |
| 2 | 24 | 226.91 | 12.24 | 5.4 |
| 3 | 24 | 458.76 | 20.82 | 4.5 |
Background Information
Interleukin-4 (IL-4), a member of the α-helical cytokine family, is produced by activated CD4 + T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, interleukin 4, Pitrakinra |