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  • KD/KO Validated

ID1 Polyclonal antibody

ID1 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 18475-1-AP

Synonyms

bHLHb24, ID, ID1, Inhibitor of DNA binding 1



Tested Applications

Positive WB detected inPC-3 cells
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18475-1-AP targets ID1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ID1 fusion protein Ag13359
Full Name inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
Calculated molecular weight 16 kDa
Observed molecular weight 20 kDa, 45-50 kDa
GenBank accession numberBC000613
Gene symbol ID1
Gene ID (NCBI) 3397
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ID (inhibitor of DNA binding) proteins contain a helix-loop-helix (HLH) motif and regulate tissue-specific transcription within several cell lineages. They do not bind DNA directly, but inhibit lineage commitment by binding basic helix-loop-helix (bHLH) transcription factors through their HLH motif. ID1 proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID proteins contribute to cell growth, senescence, differentiation, and angiogenesis. Inhibitor of differentiation or DNA binding-1 (ID1) interacts with the transactivation domain of p65 (p65TAD) both in vitro and in vivo. The molecular weight of ID1 and p65 complex is about 45-50 kDa (PMID: 22592405 ).

Protocols

Product Specific Protocols
WB protocol for ID1 antibody 18475-1-APDownload protocol
IF protocol for ID1 antibody 18475-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Exp Eye Res

Expansion and maintenance of primary corneal epithelial stem/progenitor cells by inhibition of TGFβ receptor I-mediated signaling.

Authors - Lihua Hu
ratWB

Audiol Neurootol

Id1/NR2B Receptor Pathway Regulates Rat Cochlear Sensory Epithelial Cell Survival after Radiation.

Authors - Junming Chen
  • KD Validated
humanWB

Biochim Biophys Acta

BMP-6 inhibits cell proliferation by targeting microRNA-192 in breast cancer.

Authors - Hu Fen F
humanWB

Int J Med Sci

IL-1β Promotes Stemness of Tumor Cells by Activating Smad/ID1 Signaling Pathway.

Authors - Lin Lu

Oncol Rep

High extracellular pressure promotes gastric cancer cell adhesion, invasion, migration and suppresses gastric cancer cell differentiation.

Authors - Changlei Su
ratWB,IF

J Neurosci Res

Bone morphogenetic protein 4, inhibitor of differentiation 1, and epidermal growth factor receptor regulate the survival of cochlear sensory epithelial cells.

Authors - Chen Junming J