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IFITM1-Specific Antibody

IFITM1-Specific Monoclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

5B5E2

Cat no : 60074-1-Ig

Synonyms

9 27, CD225, IFI17, IFITM1, Interferon induced protein 17, Leu 13 antigen, LEU13



Tested Applications

Positive WB detected inTF-1 cells, HepG2 cells, K-562 cells, HeLa cells, human testis tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Sample-dependent, check data in validation data gallery

Product Information

60074-1-Ig targets IFITM1-Specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IFITM1-Specific fusion protein Ag2320
Full Name interferon induced transmembrane protein 1 (9-27)
Calculated molecular weight 14 kDa
Observed molecular weight 14 kDa, 17 kDa
GenBank accession numberBC000897
Gene symbol IFITM1
Gene ID (NCBI) 8519
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFITM1(interferon induced transmembrane protein), also named DSPA2a and interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. 60074-1-Ig is a mouse monoclonal antibody which specifically recognizing IFITM1 but not IFITM2 or IFITM3.

Protocols

Product Specific Protocols
WB protocol for IFITM1-Specific antibody 60074-1-IgDownload protocol
IHC protocol for IFITM1-Specific antibody 60074-1-IgDownload protocol
IP protocol for IFITM1-Specific antibody 60074-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Clin Epigenetics

Global analysis of DNA methylation in hepatocellular carcinoma by a liquid hybridization capture-based bisulfite sequencing approach.

Authors - Fei Gao

JCI Insight

IFITM1 targets HIV-1 latently infected cells for antibody-dependent cytolysis.

Authors - Rui André Saraiva Raposo
humanWB

mBio

IFITM3 Reduces Retroviral Envelope Abundance and Function and Is Counteracted by glycoGag.

Authors - Yadvinder S Ahi
humanWB, IF

J Gen Virol

Respiratory Syncytial Virus Infection is Inhibited by Interferon-Induced Transmembrane Proteins.

Authors - Wei Zhang
  • KD Validated
WB

Viruses

IFITM Proteins Inhibit Entry Driven by the MERS-Coronavirus Spike Protein: Evidence for Cholesterol-Independent Mechanisms.

Authors - Florian Wrensch
mouseIF

PLoS One

Rapid reversal of innate immune dysregulation in blood of patients and livers of humanized mice with HCV following DAA therapy.

Authors - Matthew A Burchill