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IFNG Monoclonal antibody

IFNG Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

1C10C6

Cat no : 60101-1-Ig

Synonyms

IFG, IFI, IFN gamma, IFNG, Immune interferon

Validation Data Gallery

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  • Western Blot (WB) analysis of U-937 cells using IFNG Monoclonal antibody (60101-1-Ig)

    WB analysis of U-937 using 60101-1-Ig

    U-937 cells were subjected to SDS PAGE followed by western blot with 60101-1-Ig (IFNG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inU-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60101-1-Ig targets IFNG in WB,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IFNG fusion protein Ag8320
Full Name IFN gamma
Calculated Molecular Weight 166 aa, 19 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC070256
Gene Symbol IFNG
Gene ID (NCBI) 3458
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFNG is a soluble cytokine that is the only member of the type II class of IFNs. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.

Protocols

Product Specific Protocols
WB protocol for IFNG antibody 60101-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols