Integrin Beta 1 Polyclonal antibody
Integrin Beta 1 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 12594-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HT-1080 cells, HeLa cells |
| Positive IHC detected in | human placenta tissue, human breast cancer tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 11 publications below |
| IHC | See 2 publications below |
| IF | See 3 publications below |
Product Information
12594-1-AP targets Integrin Beta 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, sika deer |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Integrin Beta 1 fusion protein Ag3078 |
| Full Name | integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) |
| Calculated molecular weight | 88 kDa |
| Observed molecular weight | 130 kDa |
| GenBank accession number | BC020057 |
| Gene symbol | ITGB1 |
| Gene ID (NCBI) | 3688 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Integrin Beta 1 antibody 12594-1-AP | Download protocol |
| IHC protocol for Integrin Beta 1 antibody 12594-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Discov Exosomes from adipose-derived mesenchymal stem cells prevent cardiomyocyte apoptosis induced by oxidative stress. | ||
Oncotarget Annexin A4 fucosylation enhances its interaction with the NF-kB p50 and promotes tumor progression of ovarian clear cell carcinoma. | ||
J Sci Food Agric Hen Egg White Ovomacroglobulin Promotes Fibroblast Migration via Mediating Cell Adhesion and Cytoskeleton. | ||
Invest Ophthalmol Vis Sci Micro- and Nanoscale Topographies on Silk Regulate Gene Expression of Human Corneal Epithelial Cells. | ||
Oncol Lett Upregulation of nectin-4 is associated with ITGB1 and vasculogenic mimicry and may serve as a predictor of poor prognosis in colorectal cancer. | ||
Anticancer Drugs A monoclonal antibody targeting neuropilin-1 inhibits adhesion of MCF7 breast cancer cells to fibronectin by suppressing the FAK/p130cas signaling pathway. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Boyan (Verified Customer) (03-29-2019) | Good for WB - recognises a clean band around the expected molecular weight.
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