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Integrin beta-1 Antibody 14 Publications

Rabbit Polyclonal| Catalog number: 12594-1-AP

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Con: 97 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

Integrin beta-1 Antibody WB HT-1080 cells 12594-1-AP

HT-1080 cells were subjected to SDS PAGE followed by western blot with 12594-1-AP (Integrin beta-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

Integrin beta-1 Antibody IHC human placenta tissue 12594-1-AP

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12594-1-AP( Integrin beta-1 Antibody) at dilution of 1:200 (under 40x lens).

Species specificity:
human, mouse, rat, sika deer
Positive WB detected in:
HT-1080 cells, HeLa cells
Positive IHC detected in:
human placenta tissue, human breast cancer tissue, mouse kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:1000-1:4000
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Integrin beta-1 fusion protein Ag3078
Full name:
integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Calculated molecular weight:
88 kDa
Observed molecular weight:
130 kDa
GenBank accession number:
BC020057
Gene ID (NCBI):
3688
Gene symbol
ITGB1
Synonyms
CD29, FNRB, GPIIA, Integrin beta 1, ITB1, ITGB1, MDF2, MSK12, VLA 4 subunit beta, VLA BETA, VLAB

Background

Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

Integrin beta-1 Rabbit Polyclonal antibody

Boyan (Verified Customer), 03-29-2019
Applications
Western Blot,
No image submitted with review

No image submitted with review

Lot Number
Good for WB - recognises a clean band around the expected molecular weight.

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