Integrin Beta 1 Polyclonal antibody

Integrin Beta 1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12594-1-AP

Synonyms

CD29, FNRB, GPIIA, Integrin beta 1, ITB1, ITGB1, MDF2, MSK12, VLA 4 subunit beta, VLA BETA, VLAB



Tested Applications

Positive WB detected inHT-1080 cells, HeLa cells
Positive IHC detected inhuman placenta tissue, human breast cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12594-1-AP targets Integrin Beta 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat, sika deer
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Integrin Beta 1 fusion protein Ag3078
Full Name integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Calculated molecular weight 88 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC020057
Gene symbol ITGB1
Gene ID (NCBI) 3688
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.

Protocols

Product Specific Protocols
WB protocol for Integrin Beta 1 antibody 12594-1-APDownload protocol
IHC protocol for Integrin Beta 1 antibody 12594-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Death Discov

Exosomes from adipose-derived mesenchymal stem cells prevent cardiomyocyte apoptosis induced by oxidative stress.

Authors - Zhi Liu
humanWB

Oncotarget

Annexin A4 fucosylation enhances its interaction with the NF-kB p50 and promotes tumor progression of ovarian clear cell carcinoma.

Authors - Huimin Wang
humanWB

J Sci Food Agric

Hen Egg White Ovomacroglobulin Promotes Fibroblast Migration via Mediating Cell Adhesion and Cytoskeleton.

Authors - Fang Geng
humanIF

Invest Ophthalmol Vis Sci

Micro- and Nanoscale Topographies on Silk Regulate Gene Expression of Human Corneal Epithelial Cells.

Authors - Kai B Kang
humanIHC

Oncol Lett

Upregulation of nectin-4 is associated with ITGB1 and vasculogenic mimicry and may serve as a predictor of poor prognosis in colorectal cancer.

Authors - Jinxiu Zhang

Anticancer Drugs

A monoclonal antibody targeting neuropilin-1 inhibits adhesion of MCF7 breast cancer cells to fibronectin by suppressing the FAK/p130cas signaling pathway.

Authors - Fanwei Zeng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (03-29-2019)

Good for WB - recognises a clean band around the expected molecular weight.

  • Applications: Western Blot,