Integrin Beta 1 Polyclonal antibody, PBS Only

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  WB analysis using 12594-1-AP (same clone as 12594-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12594-1-AP (Integrin Beta 1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12594-1-AP (same clone as 12594-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12594-1-AP (Integrin Beta 1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 12594-1-AP (same clone as 12594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12594-1-AP (Integrin Beta 1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 12594-1-AP (same clone as 12594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12594-1-AP (Integrin Beta 1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 12594-1-AP (same clone as 12594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12594-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 12594-1-AP (same clone as 12594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12594-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 12594-1-AP (same clone as 12594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12594-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 12594-1-AP (same clone as 12594-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Integrin Beta 1 antibody (12594-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12594-1-AP (same clone as 12594-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Integrin Beta 1 antibody (12594-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Integrin Beta 1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12594-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ITGB1, CD29, FNRB, Glycoprotein IIa, GPIIA

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Product Information

12594-1-PBS targets Integrin Beta 1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Integrin Beta 1 fusion protein Ag3078
• Full Name: integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 100-130 kDa
• GenBank Accession Number: BC020057
• Gene Symbol: Integrin beta 1
• Gene ID (NCBI): 3688
• ENSEMBL Gene ID: ENSG00000150093
• RRID: AB_2130085
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P05556
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Integrin beta-1 (ITGB1), also named as CD29, is a single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.