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Kv1.2 Polyclonal antibody

Kv1.2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 14235-1-AP

Synonyms

HBK5, HK4, HUKIV, KCNA2, KV1.2, MK2, NGK1, RBK2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Kv1.2 Polyclonal antibody (14235-1-AP)

    WB analysis using 14235-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14235-1-AP (Kv1.2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of multi-cells/tissue using Kv1.2 Polyclonal antibody (14235-1-AP)

    WB analysis of multi-cells/tissue using 14235-1-AP

    WB result of anti-KCNA2 (14235-1-AP) in different cell and tissue lysates.

  • Western Blot (WB) analysis of human heart tissue using Kv1.2 Polyclonal antibody (14235-1-AP)

    WB analysis of human heart using 14235-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 14235-1-AP (Kv1.2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using Kv1.2 Polyclonal antibody (14235-1-AP)

    IP experiment of HeLa using 14235-1-AP

    IP result of anti-Kv1.2 (IP:14235-1-AP, 4ug; Detection:14235-1-AP 1:500) with HeLa cells lysate 5200ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Kv1.2 Polyclonal antibody (14235-1-AP)

    IHC staining of mouse brain using 14235-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14235-1-AP (Kv1.2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Kv1.2 Polyclonal antibody (14235-1-AP)

    IHC staining of mouse brain using 14235-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14235-1-AP (Kv1.2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA549 cells, multi-cells/tissue, human heart tissue, HeLa cells, HepG2 cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14235-1-AP targets Kv1.2 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Kv1.2 fusion protein Ag5477
Full Name potassium voltage-gated channel, shaker-related subfamily, member 2
Calculated Molecular Weight 499 aa, 57 kDa
Observed Molecular Weight 66-75 kDa
GenBank Accession NumberBC043564
Gene Symbol KCNA2
Gene ID (NCBI) 3737
RRIDAB_2280576
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Kv1.2 antibody 14235-1-APDownload protocol
IHC protocol for Kv1.2 antibody 14235-1-APDownload protocol
IP protocol for Kv1.2 antibody 14235-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols