Kv1.2 Polyclonal antibody

Kv1.2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14235-1-AP

Synonyms

HBK5, HK4, HUKIV, KCNA2, KV1.2, MK2, NGK1, RBK2



Tested Applications

Positive WB detected inA549 cells, multi-cells/tissue, human heart tissue, HeLa cells, HepG2 cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14235-1-AP targets Kv1.2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Kv1.2 fusion protein Ag5477
Full Name potassium voltage-gated channel, shaker-related subfamily, member 2
Calculated molecular weight 499 aa, 57 kDa
Observed molecular weight 66-75 kDa
GenBank accession numberBC043564
Gene symbol KCNA2
Gene ID (NCBI) 3737
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Kv1.2 antibody 14235-1-APDownload protocol
IHC protocol for Kv1.2 antibody 14235-1-APDownload protocol
IP protocol for Kv1.2 antibody 14235-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols