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LIN9 Polyclonal antibody

LIN9 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 17882-1-AP

Synonyms

BARA, BARPsv, hLin 9, HuLin 9, Lin 9, lin 9 homolog (C. elegans), LIN9, pRB associated protein, Protein lin 9 homolog, TGS, TGS1, TUDOR gene similar protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LIN9 Polyclonal antibody (17882-1-AP)

    WB analysis using 17882-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17882-1-AP (LIN9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using LIN9 Polyclonal antibody (17882-1-AP)

    IP experiment of HEK-293 using 17882-1-AP

    IP result of anti-LIN9 (IP:17882-1-AP, 4ug; Detection:17882-1-AP 1:1000) with HEK-293 cells lysate 1100ug.

Tested Applications

Positive WB detected inMDA-MB-231 cells, NIH/3T3 cells, mouse testis tissue, rat testis tissue
Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

17882-1-AP targets LIN9 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIN9 fusion protein Ag11889
Full Name lin-9 homolog (C. elegans)
Calculated Molecular Weight 558 aa, 64 kDa
Observed Molecular Weight55-65 kDa
GenBank Accession NumberBC045625
Gene Symbol LIN9
Gene ID (NCBI) 286826
RRIDAB_10859819
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIN9, also named as BARA or TGS, is a 542 amino acid protein, which belongs to the lin9 family. LIN9 localizes in the nucleus and is expressed in thymus and testis. LIN9 acts as a tumor suppressor and inhibits DNA synthesis. LIN9 plays a role in the expression of genes, which required for the G1/S transition.

Protocols

Product Specific Protocols
WB protocol for LIN9 antibody 17882-1-APDownload protocol
IP protocol for LIN9 antibody 17882-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci China Life Sci

PMID: 31420851

Authors - Hongna Lai
View Article
humanWB

EMBO Mol Med

Interactions between BRD4S, LOXL2, and MED1 drive cell cycle transcription in triple-negative breast cancer

Authors - Laura Pascual-Reguant
View Article
mouseWB

FEBS Open Bio

The dual role of the DREAM/G2M pathway in non-tumorigenic immortalization of senescent cells

Authors - Jie Tian
View Article