Filter: AllWBIHCIF/ICC

WB analysis of SW 1990 using 66303-1-Ig
SW 1990 cells were subjected to SDS PAGE followed by western blot with 66303-1-Ig (LIPH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of BxPC-3 using 66303-1-Ig
BxPC-3 cells were subjected to SDS PAGE followed by western blot with 66303-1-Ig (LIPH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 66303-1-Ig
COLO 320 cells were subjected to SDS PAGE followed by western blot with 66303-1-Ig (LIPH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas using 66303-1-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66303-1-Ig (LIPH Antibody) at dilution of 1:500 (under 10x lens).
IHC staining of human pancreas using 66303-1-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66303-1-Ig (LIPH Antibody) at dilution of 1:500 (under 40x lens).
IHC staining of human colon cancer using 66303-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66303-1-Ig (LIPH Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 66303-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66303-1-Ig (LIPH Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of BxPC-3 using 66303-1-Ig
Immunofluorescent analysis of (-20℃ Ethanol ) fixed BxPC-3 cells using 66303-1-Ig(LIPH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

LIPH Monoclonal antibody

Name: LIPH Monoclonal antibody
Brand: Proteintech
SKU: 66303-1-Ig

LIPH Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66303-1-Ig

Clone No.1F5D10

Publications(1)

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

AH, Lipase member H, lipase, member H, LIPH, LPD lipase related protein, LPDLR, mPA PLA1, mPA PLA1 alpha, MPAPLA1, Phospholipase A1 member B, PLA1B

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	SW 1990 cells, BxPC-3 cells, COLO 320 cells
Positive IHC detected in	human pancreas tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	BxPC-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IF	See 1 publications below

Product Information

66303-1-Ig targets LIPH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity	human, pig
Cited Reactivity	human
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag24009 Product name: Recombinant human LIPH protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 103-451 aa of BC064941 Sequence: VVVVDWNRGATTLIYTHASSKTRKVAMVLKEFIDQMLAEGASLDDIYMIGVSLGAHISGFVGEMYDGWLGRITGLDPAGPLFNGKPHQDRLDPSDAQFVDVIHSDTDALGYKEPLGNIDFYPNGGLDQPGCPKTILGGFQYFKCDHQRSVYLYLSSLRESCTITAYPCDSYQDYRNGKCVSCGTSQKESCPLLGYYADNWKDHLRGKDPPMTKAFFDTAEESPFCMYHYFVDIITWNKNVRRGDITIKLRDKAGNTTESKINHEPTTFQKYHQVSLLARFNQDLDKVAAISLMFSTGSLIGPRYKLRILRMKLRSLAHPERPQLCRYDLVLMENVETVFQPILCPELQL Predict reactive species
Full Name	lipase, member H
Calculated Molecular Weight	51 kDa
Observed Molecular Weight	50-55 kDa
GenBank Accession Number	BC064941
Gene Symbol	LIPH
Gene ID (NCBI)	200879
RRID	AB_2881686
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q8WWY8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIPH(Lipase member H) is also named as LPDLR, MPAPLA1, PLA1B. It is a phosphatidic acid-selective phospholipase A1 (PLA1) that produces 2-acyl lysophosphatidic acid (LPA) and belongs to the AB hydrolase superfamily and Lipase family.LIPH has a 12-residue lid region, which likely covers a catalytic pocket, and 4 potential N-linked glycosylation sites. Defects in LIPH are the cause of hypotrichosis type 7 (HYPT7).

Protocols

Product Specific Protocols
IF protocol for LIPH antibody 66303-1-Ig	Download protocol
IHC protocol for LIPH antibody 66303-1-Ig	Download protocol
WB protocol for LIPH antibody 66303-1-Ig	Download protocol