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LIPH Monoclonal antibody

LIPH Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1F5D10

Cat no : 66303-1-Ig

Synonyms

AH, Lipase member H, lipase, member H, LIPH, LPD lipase related protein, LPDLR, mPA PLA1, mPA PLA1 alpha, MPAPLA1, Phospholipase A1 member B, PLA1B

Validation Data Gallery

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  • Western Blot (WB) analysis of SW 1990 cells using LIPH Monoclonal antibody (66303-1-Ig)

    WB analysis of SW 1990 using 66303-1-Ig

    SW 1990 cells were subjected to SDS PAGE followed by western blot with 66303-1-Ig (LIPH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using LIPH Monoclonal antibody (66303-1-Ig)

    WB analysis of BxPC-3 using 66303-1-Ig

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 66303-1-Ig (LIPH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using LIPH Monoclonal antibody (66303-1-Ig)

    WB analysis of COLO 320 using 66303-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66303-1-Ig (LIPH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using LIPH Monoclonal antibody (66303-1-Ig)

    IHC staining of human pancreas using 66303-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66303-1-Ig (LIPH Antibody) at dilution of 1:500 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using LIPH Monoclonal antibody (66303-1-Ig)

    IHC staining of human pancreas using 66303-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66303-1-Ig (LIPH Antibody) at dilution of 1:500 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LIPH Monoclonal antibody (66303-1-Ig)

    IHC staining of human colon cancer using 66303-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66303-1-Ig (LIPH Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LIPH Monoclonal antibody (66303-1-Ig)

    IHC staining of human colon cancer using 66303-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66303-1-Ig (LIPH Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using LIPH Monoclonal antibody (66303-1-Ig)

    IF Staining of BxPC-3 using 66303-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed BxPC-3 cells using 66303-1-Ig(LIPH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inSW 1990 cells, BxPC-3 cells, COLO 320 cells
Positive IHC detected inhuman pancreas tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

66303-1-Ig targets LIPH in WB, IHC, IF, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen LIPH fusion protein Ag24009
Full Name lipase, member H
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC064941
Gene Symbol LIPH
Gene ID (NCBI) 200879
RRIDAB_2881686
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIPH(Lipase member H) is also named as LPDLR, MPAPLA1, PLA1B. It is a phosphatidic acid-selective phospholipase A1 (PLA1) that produces 2-acyl lysophosphatidic acid (LPA) and belongs to the AB hydrolase superfamily and Lipase family.LIPH has a 12-residue lid region, which likely covers a catalytic pocket, and 4 potential N-linked glycosylation sites. Defects in LIPH are the cause of hypotrichosis type 7 (HYPT7).

Protocols

Product Specific Protocols
WB protocol for LIPH antibody 66303-1-IgDownload protocol
IHC protocol for LIPH antibody 66303-1-IgDownload protocol
IF protocol for LIPH antibody 66303-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Genet Med

Loss-of-function variants in C3ORF52 result in localized autosomal recessive hypotrichosis.

Authors - Liron Malki
View Article